Tissue-engineered blood vessels

They also seeded autologous vSMC and ECs obtained from ovine carotid arteries to study autologous tissue-engineering blood vessels in the descending aorta of juvenile sheep. They found that after three months implantation, grafts were fully patent, without dilatation, occlusion, or intimal thickening. A continuous luminal EC layer was formed. However, after six months ... 

S. Kashiwagi, Y. Izumi, T. Gohongi, Z.N. Demou, L. Xu, P.L. Huang, D.G. Buerk, L.L. Munn, R K. Jain, and D. Fukumura, NO mediates mural cell recruitment and vessel morphogenesis in murine melanomas and tissue-engineered blood vessels. J. Clin. Invest. 115, 1816-1827 (2005). 

Other attempts at tissue engineering blood vessels have been made by constructing the vessels ex vivo directly from the cellular components. This may be accomplished by culturing a sheet of human vascular smooth muscle cells in collagen and placing it within a lumenal support to produce tire media. A fibroblast sheet is then similarly cultured and placed about tire media to form the adventitia. Endothelial cells are later seeded into the lumen of the vessel, thus forming a mechanically soimd, three-dimensional vessel. 

L Heureux N, Dusserre N, Konig G, Victor B, Keire P, Wight TN, et al. Human tissue-engineered blood vessels for adult arterial revascularization. Nat Med 2006 12 361-5. 

L Heureux N, McAllister TN, de la Fuente LM. Tissue-engineered blood vessel for adult... 

Quint C, Kondo Y, Manson RJ, Lawson JH, Dardik A, Niklason LE. DeceUular-ized tissue-engineered blood vessel as an arterial conduit. Proc Natl Acad Sci USA 2011 108(22) 9214-9. 

Quint C, Arief M, Muto A, Dardik A, Niklason LE. Allogeneic human tissue-engineered blood vessel. J Vase Surg 2012 55(3) 790-8. 

The physical characteristics of AM have been investigated as a potential biomaterial matrix in the engineering of blood vessels. Lee described using glutaraldehyde cross-linked AM tubes as a scaffold biomaterial for tissue-engineered blood vessel... 

Lee PH, Tsai SH, Kuo L, Hwang CY, Yang VC, Kuo CY, et al. A prototype tissue engineered blood vessel using amniotic membrane as scaffold. Acta Biomater 2012 8 3342-8. 

Xiang, P., Li, M., Zhang, C.Y., Chen, D.L., and Zhou, Z.H. (2011) Cytocom-patibility of electrospun nano fiber tubular scaffolds for small diameter tissue engineering blood vessels. Int J. Biol. Macromol, 49 (3), 281—288. 

Hoenig MR, Campbell GR, Rolfe BE and Campbell JH. Tissue-engineered blood vessels. Alternative to autologous grafts Arteriose. Thromb. Vase. Biol. 25 1128,2005. 

Greisler HP, Gosselin C, Ren D, Kang SS, Kim DU. Biointeractive polymers and tissue engineered blood vessels. Biomaterials 17 329-336,1996. 

Konig G, McAllister TN, Dusserre N, Garrido SA, lyican C, Marini A, FioriUo A, Avila H, Wystrychowski W, Zagalski K, Maruszewski M, Jones AL, Cierpka L, de la Fuente LM, L Heureux N. Mechanical properties of completely autologous human tissue engineered blood vessels compared to human saphenous vein and mammary artery. Biomaterials 30 1542-1550,2009. 

Yao L, Liu J, Andreadis ST. Composite fibrin scaffolds increase mechanical strength and preserve contractility of tissue engineered blood vessels. Pharm Res 2008 25 1212-21. 

SeHktar, D., Nerem, R.M., and Galis, Z.S., Mechanical strain-stimulated remodeling of tissue-engineered blood vessel constructs, Tissue Eng., 9,657,2003. 

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