Fibrin, composition

Losi, P, Briganti, E., Magera, A., SpiUer, D., Ristori, C., Battolla, B., Balderi, M., Kull, S., Balbarini, A., Di Stefano, R., Soldani, G., 2010. Tissue response to poly(ether)ure-thane-polydimethylsiloxane-fibrin composite scaffolds for controlled delivery of pro-an-giogenic growth factors. Biomaterials 31, 5336-5344. 

Several studies have shown that microbial cellulose can be molded into tubular form with diameter < 6 mm. Klemm et al. [101] prepared a microbial cellulose tube having 1 mm diameter and 5 mm length with a wall thickness of 0.7 mm. The tensile strength of the material was foxmd to be comparable to that of normal blood vessels (800 mN) and is employed as blood vessel to replace part of the carotid artery. Alter four weeks, the microbial cellulose/carotid artery complex was covered with connective tissue. The in-vivo bicompatibility tests show that microbial cellulose can be used as a replacement blood vessel. Recently, Brown et al [102] have prepared small tubes of microbial cellulose-fibrin composites treated with glutaraldehyde in order to crosslink the polymers and allow a better match of the mechanical properties with those of native small-diameter blood vessels. 

Losi, F, E. Briganti, A. Magera et al, 2010. Tissue response to poly(ether)urethane-polydimethylsiloxane-fibrin composite scaffolds for controlled delivery of pro-angiogenic growth factors. Biomaterials 31 5336-44. 

Pankajakshan, D., Philipose, L.P., Palakkal, M., Krishnan, K., Krishnan, L.K.J. Development of a fibrin composite-coated poly(e-caprolactone) scaffold for potential vascular tissue engineering apphcations. J. Biomed. Mater. Res., Part B 87B, 570-579 (2008)... 

Scaffolds can be made from natural or synthetic materials. Such materials fall under the category of biomaterials. A biomaterial can be considered a single element or compound, which is a composite or mixture of elements, and is synthesized or derived to be used in the body to preserve, restore, or augment the structure or function of the body. Examples of natural materials for scaffold construction are extracellular matrix, collagen, fibrin, and polysaccharides (e.g., chitosan or glycosaminoglycans). Natural materials, unless they are obtained from the patient who receives the neo-organ implant, will cause an immunogenic response. This is not always the case with synthetic materials. 

The composition of the cotton threaded thrombus shows a composition of fibrin together with tightly aggregated and distorted erythrocytes, thus being in accordance with human deep vein thrombosis structure. Non-occlusive thrombus formation has been successfully inhibited by heparins, prothrombinase complex inhibitors and thrombin inhibitors (Hollenbach et al. 1994,1995). 

Using this procedure, Consden and Stanier were able to show that a hydrolyzate of Group A hemolytic streptococci contained hexosamine, rhamnose, ribose, and glucose and, also, that a hydrolyzate of purified, human fibrin contained uronic acid, hexosamine, mannose, and galactose. A similar combination of techniques has been employed by Woodin in a study of the composition of a comeal mucopolysaccharide. It is of interest that the conditions of acidic hydrolysis necessary for releasing the n-glucos-amine moiety from heparin also result in destruction of the concomitantly released n-glucuronic acid. Traces of the intact uronic acid may be revealed by zone electrophoresis of the hydrolyzates. ... 

General surgery Skin repair template Sutures Adhesives and sealants Silicone-collagen composite Silk, nylon, poly(glycolide-co-lactide) Cyanoacrylate, fibrin... 

Composition Platelets Fibrin RBCs and WBCs Lacks platelets Fibrin RBCs and WBCs... 

Fig. 19 Fabricating procedure of the composite construct by filling BMSCs, TMC/DNA complexes, and fibrin gel into a PLGA sponge. Bottom left Chemical stmcture of TMC. pDNA-TGF-pi was used in the in vivo experiment [207]...

Fig. 2. Fibrin zymography of PA standards. (A) Fibrin zymogram demonstrating electrophoretic migration of uPA (45-kDa), tPA (70-kDa), and higher molecular weight tPA/PAI-1 complex (110-kDa). Fibrin indicator gels were stained with amido black (see Section 3.5). Samples 1-5 correspond to PAs and PAIs of various compositions as described (R4, R5). (B) Calibration curve obtained with PA standards. Linear regression analysis was performed and correlation coefficient (r) is shown (r = 1.000, perfect correlation).

In the table above, as Witte peptone comes from a transformation of fibrin, we have given the composition of the products obtained after hydrolysis of the latter with acid. These ana lytical data show beyond doubt that plastein is a very complex substance, approaching closely the albiuninoids in composition. Moreover, of all the products which are found in peptone, only the fraction precipitable by phosphotungstic acid can give rise to a plastein of composition analogous to that given above. 

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