Thyroid hormone receptor binding assay

Thyroid Hormone Receptor (TR) can bind to the LTR in vivo independently of its ligand and regulates promoter activity. ChIP assays with anti-acetylated-histone antibodies revealed that unliganded TR reduce the local histone acetylation levels at the HIV-1 LTR, while thyroid hormone treatment reverses this induction (Hsia and Shi, 2002). Accordingly, unliganded TR recruits co repressors and at least one HDAC (Hsia and Shi, 2002). 

Jeyakumar, M. and Katzenellenbogen, J.A. 2009. A dual-acceptor time-resolved Forster resonance energy transfer assay for simultaneous determination of thyroid hormone regulation of corepressor and coactivator binding to the thyroid hormone receptor. Anal. Biochem. 386, 73-78. 

Polybrominated Diphenyl Ethers. Results of in vitro estrogen receptor and thyroid hormone transport protein binding assays and in vivo studies of thyroid hormone homeostasis indicate that PBDEs have the potential to disrupt normal endocrine function. 

Bockmann J, Winter C, Wittkowski W, Kreutz MR, Bockers TM (1997) Cloning and expression of a brain-derived TSH receptor. Biochem Biophys Res Commun 238 173-1780 Castagiola A, Swillens S, Niccoli P, Dumont JE, Vassart G, Ludgate M (1992) Binding assay for thyrotropin receptor autoantibodies using the recombinant receptor protein. J Clin Endocrinol Metab 75 1540-1544 Cole ES, Lee K, Lauziere K et al. (1993) Recombinant human thyroid stimulating hormone development of a biotech-... 

Jeyakumar and Katzenellenbogen developed a dual-acceptor assay to monitor a nuclear receptor (thyroid hormone - retinoid X receptor heterodimer) regulation by measuring both coactivator and corepressor binding by using fluorescein and Cy-5 as acceptors for a terbium chelate-labeled response element-bound receptor complex [47]. 

---