Time-resolved Forster resonance

Time-Resolved Forster Resonance Energy Transfer (TR-FRET)... 

Jeyakumar, M. and Katzenellenbogen, J.A. 2009. A dual-acceptor time-resolved Forster resonance energy transfer assay for simultaneous determination of thyroid hormone regulation of corepressor and coactivator binding to the thyroid hormone receptor. Anal. Biochem. 386, 73-78. 

Norskov-Lauritsen, L., Thomsen, A. R., Brauner-Osbome, H. (2014). Gprotein-coupled receptor signahng analysis using homogenous time-resolved Forster resonance energy transfer (HTRF(R)) technology. International Journal of Molecular Sciences, 15(2), 2554-2572. 

TR-FRET Time-resolved Forster resonance energy transfer... 

Hildebrandt N, Charbonniere LJ, Lohmannsroben HG (2007) Time-resolved analysis of a highly sensitive forster resonance energy transfer immunoassay using terbium complexes as donors and quantum dots as acceptors. J Biomed Biotechnol 2007 79169... 

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Forster s theory is also behind the FRET method (Forster resonance energy transfer or fluorescence resonance energy transfer). In this case, excitation takes place with a narrow laser pulse, corresponding to the lowest excited state of a chromo-phore. The rate of EET is determined by time-resolved fluorescence. 

Time resolved fluorescence measurements have become an important tool in applied fluorescence spectroscopy. Recently, it has been pointed out that the controlled manipulation of fluorescence decay rates opens a new dimension in applied fluorescence spectroscopy. The fluorescence decay rate depends on two independent contributions, the pure rachative rate and the nonradiative rate. The latter one can be influenced by the well known Forster-type resonant energy transfer processes, while the radiative rate can be changed if the molecules are embedded or close to media comprising a dielectric constant markedly different from vacuum. Especially metal nanostructures have been used to alter both decay paths of fluorescent molecules. Apart from a change of those two rates, the absorption cross-section might also be altered. 

Luminescence resonance energy transfer (LRET) proceeds by radiationless dipole-dipole coupled energy transfer from an excited luminophore through space to another lumino-phore [30]. There are several criteria that must be met for LRET. First, the emission band of the excited luminophore donor must overlap the absorbance band of the acceptor luminophore. The distance between the donor and acceptor molecules can be measured by using Equation 8.2 [31]. In Equation 8.2, the efficiency of energy transfer is E, tda is the time-resolved luminescence lifetime of the donor-acceptor pair, tq is the lifetime of the donor, Rq is the distance for 50% energy transfer to occur (or Forster distance), and r is the calculated distance between the donor and acceptor. 

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