Thrombin receptor-activating peptide

Tagging and recovery of associated proteins, as in RNA-TRAP also thrombin receptor activation peptide Thyroid hormone response elements Thyrotropin-releasing hormone... 

Goodwin CA, Wheder-Jbnes CPD, Kakkar W, Deadman JJ, Authi KS, Scully MF Thrombin receptor activating peptide does not stimulate platdd procoagulant activity. Biochem Biophys Res Common 202 321,1994... 

Lasne D, Donato J, Fdd H, Rendu F Different abilities of thrombin receptor activating peptide and thrombin to induce platdd caldum rise and full rdease reaction. Thromb Haemost 74 1323,1995... 

Structure-activity studies with thrombin receptor-activating peptides (TRAPs). 

TRAP, thrombin receptor-activating peptide PI4, SFLLRNPNDKYEPF P7, SFLLRNP P7-NH2, SFLLRI -NH2 P5, SFLLR PS-NHz, SFLLR-NH2 P5VR-NH2, SFLVR-NHj Cha, cyclohexylalanyl Mpr, mercaptopropionyl LM, gastric longitudinal muscle. 

All interactions between UFH and platelets are complex and only partially elucidated, but it is known that heparin itself stimulates platelets via a platelet-binding domain of heparin (14). In the therapeutic range, UFH induces the release of P-selectin and activates GPIIb/llla receptors when adenosine diphosphate (ADP) or thrombin receptor agonist peptide stimulates platelet responsibility, and then enhances platelet aggregation (15,16). Even in healthy individuals, agonist-induced platelet aggregation is often enhanced when heparin is added. 

Xiao Z, Theroux R Clopidogrel inhibits platelet-leukocyte interactions and thrombin receptor agonist peptide-induced platelet activation in patients with an acute coronary syndrome. J Am Coll Cardiol 2004 43 1 982-1988. 

Lau L-F, PumigliaK, Cote YP, Feinstein MB Thrombin-receptor agonist peptides, in contrast to thrombin itsdf, are not fiill agonists for activation and signal transduction in human platdets in the absence of platdd-derived secondary mediators. Biochem J 303 391,1994... 

Kramer RM, Roberts EF, Hydop PA, Utteriiack BG, Hui KY, Jakubowski JA Differential activation of thrombin receptor agonist peptide in human platdets. J Biol Chem 270 14816,1995... 

Kramer RM, Roberts EF,Hyslop PA UtterbackBG,HuiKY, and Jakubowski JA (1995). Differendal activation of cytosolic diospholipase A2 (cPLA2) by thrombin and thrombin receptor agonist peptide in human platelets. Evidence for activation of cPLA2 indepoident of the mitOgen-activated protein kinases ERKl/2. J. Biol. Chem. 270,14816-4823. 

At this point, we had assembled a preliminary model of the human thrombin receptor that contained all three extracellular loops linked to the seven TM domains, as well as the N-terminus out to residue 75. Docking studies with agonist peptide SFLLRN, and additional mutagenesis experiments, were carried out in parallel. Measurement of the effects of site-specific mutations in human PAR-1 identified several key amino acids of the receptor as being important for activation (Table 1),... 

Cook NS, Zerwes H-G, Tapparelli C et al. (1993) Platelet aggregation and fibrinogen binding in human, rhesus monkey, guinea-pig, hamster and rat blood activation by ADP and thrombin receptor peptide and inhibition by glycoprotein Ilb/IIIa antagonists. Thromb Haemostasis 70 531-539... 

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