Fibrinogen binding

OXIN O partially inhibited ADP-induced platelet aggregation in vitro. Flow cytometry analysis of PRP showed that OXINO decreased ADP-induced CD62P expression and fibrinogen binding on platelets [45]. 

Benzodiazepines reduce fibrinogen binding and platelet aggregation Central Nervous System Acts as sedative-hypnotic, opens ion channel, chloride ion... 

Basani, R. B., D Andrea, G., Mitra, N., Vilaire, G., Richberg, M., Kowalska, M. A., Bennett, J. S., and Poncz, M. (2001). RGD-containing peptides inhibit fibrinogen binding to platelet alpha(IIb)beta 3 by inducing an allosteric change in the amino-terminal portion of alpha (lib). / Biol. Chem. 276, 13975-13981. 

VII. Fibrinogen Binding to Integrins in Platelet Aggregation and Other Cellular Interactions... 

Fig. 33.1. Structure of DNA aptamers for recognition fibrinogen (A) [15,29] and heparin binding sites (B) [17] of thrombin. The non-canonical hydrogen bonds between guanines are shown by dotted lines. In the case of structure (A), the spacer composed of 15 T chain terminated by thiol group at the end of hydrophobic spacer is shown [34]. (C) Structure of RNA aptamer against fibrinogen binding site of thrombin [23].

Use, for example, 32-mer DNA aptamer modified by biotin at 3 end of the following sequence 3 -BIO-GGG TTT TCA CTT TTG TGG GTT GGA CGG GAT GG - 5. This aptamer has at its 5 end typical motif with high affinity to the heparin-binding site of the thrombin [1], (Please note that aptamer that selectively bind the fibrinogen-binding site of thrombin has a different structure—see Chapter 33 in CD accompanying this book). The aptamer can be purchased from Thermo Electron Corporation, Germany. 

Several potent inhibitors of fibrinogen binding to gpIIb/IIIa are now known, and some have proven to be effective antithrombotic agents. These inhibitors tend either to be small cyclic peptide mimetics, in which the conformation of an RGD sequence is constrained by macrocyclization, or they are non-peptidic molecules containing functional group mimetics of the Arg and Asp side chains, held in the correct geometry for interaction with the receptor. Examples of both classes are shown in... 

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