Thrombin-Induced Clot Formation in Canine Coronary Artery

Thrombin-Induced Clot Formation in Canine Coronary Artery 

In the initial study described by Gold et al. (1984), recombinant t-PA was characterized for its ability to lyse 2-hour-old thrombi. Tissue plasminogen activator was infused at doses of 4.3, 10, and 25 (ig/kg/min, i.v, and resulted in reperfusion times of 40, 31, and 13 minutes, respectively. Thus, in this model of canine coronary thrombosis, t-PA exhibited dose-dependent coronary thrombolysis. Furthermore, it is possible to study the effect of different doses of t-PA on parameters of systemic fibrinolytic activation, such as fibrinogen, plasminogen, and a2-antiplasmin, as well as to assess myocardial infarct size. For example, Kopia et al. (1988) demonstrated that SK elicited dose-dependent thrombolysis in this model. 

Subsequently, Gold et al. (1986, 1988) modified the model to study not only reperfusion, but also acute reocclusion. Clinically, reocclusion is a persistent problem after effective coronary thrombolysis, which is reported to occur in 15 15 % of patients (Goldberg 

Thrombin-Induced Rabbit Femoral Artery Thrombosis Localized thrombosis can also be produced in rabbit peripheral blood vessels such as the femoral artery by injection of thrombin, calcium chloride, and fresh blood via a side branch (Shebuski et al. 1988). 

Collen D, Stassen JM, Verstraete M (1983) Thrombolysis with human extrinsic (tissue-type) plasminogen activator in rabbits with experimental jugular vein thrombosis. J Clin Invest 71 368-376 

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