Three-dimensional cell growth

Luo, Y. Shoichet, M.S. A photolabile hydrogel for guided three-dimensional cell growth and migration. Nat. Mater. 2004, 3, 249-253. 

Schlie, S., Ngezahayo, A., Ovsianikov, A., Fabian, T., Kolb, H.A., Haferkamp, H., and Chichkov, B.N. (2007) Three-dimensional cell growth on structures fabricated from ORMOCER (R) by two-photon polymerization technique. 

Figure 21.8 SEM images and CCK-8 assay (a) glial cell adhesion and proliferation on films and scaffolds of PUCL-n/t-PEG and PCL cultured at 48 h white arrow, three-dimensional cell growth in PUCL-uft-PEG scaffold (b) CCK-8 assays of glial cell viability on PUCL-a/t-PEG, PUCL-ran-PEG and PCL-based films and scaffolds.

Banu, N., M. Rosenzweig, H. Kim, J. Bagley, and M. Pykett. 2001. Cytokine-augmented culture of haematopoietic progenitor cells in a novel three-dimensional cell growth matrix. Cytokine 13(6) 349-58. 

Beaty, C.E. and W.M. Saltzman, Controlled growth factor delivery induces differential neurite outgrowth in three-dimensional cell cultures. Journal of Controlled Release, 1993, 24, 15-23. 

Dangles V, Femenia F, Laine V, Berthelemy M, Le Rhun D, Poupon MF, Levy D, Schwartz-Cornil 1. Two- and three-dimensional cell structures govern epidermal growth factor survival function in human bladder carcinoma cell Unes. Cancer Res 1997 57 3360-3364. 

For all of the above approaches, the transfer of an artificial pattern onto a cell growth substrate is commonly accomplished by photolithography or laser director writing. Recently even ink-jet printers have been utilized for pattern transfer. Using a layer-by-layer approach, a microflu-idic chip has been able to generate three-dimensional cell patterns [2]. 

Howes AL, Chiang GG, Lang ES et al (2007) The phosphatidylinositol 3-kinase inhibitor, PX-866, is a potent inhibitor of cancer cell motility and growth in three-dimensional cultures. Mol Cancer Ther 6 2505-2514... 

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