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Thiophilic ligands

Fig. 1 Structure of the thiophilic ligand created by the reaction of 2-mercaptoethanol with divinyl sulfone-activated agarose. Immunoglobulins bind to the adjacent sulfone and thioether groups. Fig. 1 Structure of the thiophilic ligand created by the reaction of 2-mercaptoethanol with divinyl sulfone-activated agarose. Immunoglobulins bind to the adjacent sulfone and thioether groups.
TABLE 10 Main Thiophilic Ligand Structures Described for the Separation of Antibody... [Pg.586]

Thiophilic ligands [ 190]. [ 191 ] form a separate class of group-specific ligands that possess high selectivity with respect to immunoglobulins. They are low-molecular compounds, and contain sul-fone and thioether groupings (see below), which show a strong affinity for mono- and polyclonal antibodies in the presence of ammonium sulfate (ca. 1 mol/L). [Pg.320]

Complex aminoacid sequences structure Thiophilic like ligands Hydrophobic charge transfer effect... [Pg.575]

Heterocyclic structures acting as thiophilic sorbents for the separation of immunoglobulins have also been described. In 1988 Porath and Oscarsson157 described several structures involving thiophilic electron-donor-acceptor mechanism. Pyridyl sulfide and phenyl sulfide molecules were attached to agarose beads and then used for the separation of plasma proteins. All these ligands adsorbed preferably immunoglobulins. [Pg.584]

More recently thiophilic heterocyclic ligands have been claimed as improved compounds for the adsorption of antibodies in a salt independent manner.162,163... [Pg.585]

All studies on thiophilic adsorption of immunoglobulins make evident that the adsorption is improved when sulfur atoms are present in the ligand. The specificity for IgG is not extremely high, but can be modulated by the... [Pg.585]

Ligands selected for HCIC applied to purification of antibodies, exhibit a mild hydrophobic effect, a thiophilic effect and an ionic charge at the ligand head. Table 11 shows some ligands selected for the separation of immunoglobulins by HCIC. HCIC demonstrated its effectiveness to adsorb immunoglobulins of different classes. IgG, IgM were adsorbed on the matrix in similar conditions as per protein A and protein A mimetic ligands, respectively, described in Sections V.F.ii and V.F.x. [Pg.587]

Knudsen, K. L., Hansen, M. B., Henriksen, L. R., Andersen, B. K., and Lihme, A. (1992). Sulfone-aromatic ligands for thiophilic adsorption chromatography Purification of human and mouse immunoglobulins. Anal. Biochem. 201, 170-177. [Pg.629]

Schwarz A. (1996). Five-membered mercaptoheterocyclic ligands for thiophilic adsorption chromatography. J. Mol. Recognition 6, 672-674. [Pg.629]

Scholz, G. H., Vieweg, P., Leistner, S., and Huse, K. (1998). Salt-independent binding of antibodies from human serum to thiophilic heterocyclic ligands. J. Chromatogr. B 709, 189-196. [Pg.629]

Bakhshpour M, Bereli N, Senel S (2014) Preparation and characterization of thiophilic cryogels with 2-mercapto ethanol as a ligand for IgG purification. Colloid Surface B 113 261-268... [Pg.279]

See other pages where Thiophilic ligands is mentioned: [Pg.585]    [Pg.706]    [Pg.881]    [Pg.321]    [Pg.585]    [Pg.706]    [Pg.881]    [Pg.321]    [Pg.95]    [Pg.259]    [Pg.143]    [Pg.54]    [Pg.148]    [Pg.92]    [Pg.140]    [Pg.122]    [Pg.584]    [Pg.585]    [Pg.629]    [Pg.4633]    [Pg.29]    [Pg.1082]    [Pg.115]    [Pg.568]    [Pg.103]    [Pg.42]    [Pg.92]    [Pg.4632]    [Pg.195]    [Pg.768]    [Pg.65]    [Pg.54]    [Pg.378]    [Pg.38]    [Pg.173]    [Pg.299]    [Pg.43]    [Pg.24]    [Pg.38]   
See also in sourсe #XX -- [ Pg.320 ]



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Thiophilic ligand structures

Thiophilicity

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