Thin-Layer Electrophoresis of Amines

Equipment for thin-layer electrophoresis is described on p. 109. Pastuska and Teinks [23] have separated a number of amines on silica gel G layers which had been impregnated with borax. The amines listed below migrated towards the cathode and the values refer to methylamine as 1.00 ethylamine 0.69 n-butylamine 0.82 ethanol-amine 1.09 ethylenediamine 0.32 and triethanolamine 0.51. These Mq values were obtained under the following experimental conditions  

The silica gel G was shaken up with a 3% borax, solution and poured on to glass plates. After 20— 26 ruin, 5—10 xg of each of the amine hydrochlorides were applied to the still moist layer at about 7 cm from the strip on the anode side. The electrolyte consisted of a mixture of 80 ml ethanol, 30 ml distilled water and 2 g anhydrous sodium acetate which had been brought to pH 12 with 40% sodium hydroxide. A duration of 2 h is quoted at a field strength of 10 V/cm. It is important to renew the electrolyte solution for each experiment. 

Reference may be made to the combination of thin-layer electrophoresis with TLC in a two-dimensional procedure, described by Honbg- 

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