Amines thin-layer electrophoresis

Equipment for thin-layer electrophoresis is described on p. 109. Pastuska and Teinks [23] have separated a number of amines on silica gel G layers which had been impregnated with borax. The amines listed below migrated towards the cathode and the values refer to methylamine as 1.00 ethylamine 0.69 n-butylamine 0.82 ethanol-amine 1.09 ethylenediamine 0.32 and triethanolamine 0.51. These Mq values were obtained under the following experimental conditions ... 

Several analytical techniques including capillary electrophoresis, thin layer chromatography (TLC), GC, lEC, and HPLC, have been proposed for the determination of biogenic amines in various foods. Among these, RP-HPLC is considered the most suitable one. HPLC methods used for amine determination usually involve two steps amine extraction from the matrix and analytical determination. Depending on the complexity of food matrix and the selectivity of the final analytical determination, a further purification step may be necessary prior to the analytical determination. To ensure adequate sensitivity, a derivatization step is generally required before injection [282]. 

It is well known that the phenol groups (which are bonded to the aporphine ring) and the benzylic amine function are responsible for the antioxidant and free radical scavenging activities of boldine [60], In addition, the presence of hydroxyl moieties in the chemical skeleton of boldine is responsible for its lipid peroxidation inhibitory activity [61], The level of boldine in the bark of boldo is more than 6% [62], Paper electrophoresis, voltammetric method, thin-layer chromatography and gas chromatography are common analytical methods for boldine determination [63],... 

High-voltage paper electrophoresis was the first method introduced for the identification of dansylated amino acids and for the separation of peptides [1,6] subsequently, electrophoretic separations on thin layers were also reported [34,35]. These methods are, however, unsuitable for the separation of amine derivatives. TLC on silica gel hamino acid, f>eptide and amine derivatives. A large variety of solvent systems of varying polarity is now available, which allows the separation of even complex mixtures of amino acids [7,36—38] and amines [7,17,39]. Fingerprinting or identification of Dns-peptides can also be carried out on silica gel layers 29,40,41]. For the separation of Dns-peptides, electrophoresis in polyacrylamide gel and on cellogel strips was reconunended (42,43]. Alumina [44] kieselguhr (45] and cellulose thin-layers [46] have also been tried. 

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