Poly grafted surfaces

Takei, Y. G., Aoki, T., Sanui, K., Ogata, N., Sakurai, Y, and Okano, T. Dynamic contact angle measurement of temperature-responsive surfaces properties for poly(Af-isopropylacrylamide) grafted surfaces. Macromolecules, 1994, 27, 6163—6166. 

Mei Y, Wu T, Xu C, Langenbach KJ, Elliott JT, Vogt BD, Beers KL, Amis EJ, Washburn NR (2005) Tuning cell adhesion on gradient poly(2-hydroxyethyl methacrylate)-grafted surfaces. Langmuir 21 12309-12314... 

Fig. 21 A Schematics of albumin-bound poly(N-isopropylacrylamide) (PNIPAM)-grafted surface via albuminated DC iniferter. B Hypothetical action of temperature-dependent switching of protein desorption by squeezing out and mechanical motion...

Okano et al. [51] applied poly(IPAAm) to modifying the surface of commercial polystyrene1 culture dishes for bovine aortic endothelial cells as well as for rat hepatocytes. Graft polymerization of IPAAm onto the polystyrene dishes was carried out by using an electron beam. The thickness of the poly(IPAAm)-grafted layer in aqueous systems was 0.5 pm at 37 °C and 0.6 pm at 15 °C. After endothelial cells were cultured at 37 °C for 2 days, the temperature was decreased to 10 °C and the number of cells that were detached from the surface was counted. Okano et al. observed that 100% of cells were detached from the poly(IPAAm)grafted surface. 

Kikuchi A, Okuhara M, Karikusa F, Sakurai Y, Okano T. Two-dimensional manipulation of confluently cultured vascular endothelial cells using temperature-responsive poly(AT-isopropylacrylamide)-grafted surfaces. J Biomater Sci Polym Ed 1998 9(12) 1331-1348. 

Uchida K, Sakai K, Ito E, Hyeong Kwon O, Kikuchi A, Yamato M, et al. Temperature-dependent modulation of blood platelet movement and morphology on poly(A-isopropylacrylamide)-grafted surfaces. Biomaterials 2000 21 923-9. 

Surface block-graft-copolymerization, based on the photochemistry of N, AT-diethyldithiocarbamate has been applied to precisely design biocompatible and functional surfaces (patterns of immobilized heparin or proteins), as well as block-grafted surfaces on polystyrene [83]. Polystyrene surfaces have also been patterned by immobilization of poly(Af-isopropylacrylamide) by photolithography, and subsequently used for regiospecific cell attachment [84]. Surface modification of polydimethylsiloxane microfluidic devices by UV induced polymer grafting improved the stability of the electroosmotic mobility and improved electrophoretic resolution of peptides [85]. 

Combellas C, Fuchs A, Kanoufi F, Mazouzi D, Nunige S (2004) Surface modification of halogenated polymers. 6. Graft copolymerization of poly (tetrafluoroethylene) surfaces by polyacrylic acid. Polymer 45(14) 4669 675... 

Combellas C, Kanoufi F, Nunige S (2007) Surface modification of halogenated polymers. 10. Redox catalysis induction of the polymerization of vinylic monomers. Application to the localized graft copolymerization of poly(tetrafluoroethylene) surfaces by vinylic monraners. Chem Mater 19(15) 3830-3839... 

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