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Tetraploid embryo

Subheading 3.1.3, step 6 Use only confirmed ES cell clones for generating mouse lines by blastocyst injection or tetraploid embryo aggregation. Resulting mice need to be mated to Cre deleter strains 21) for knockdown activation. [Pg.319]

The methods described in this chapter require the standard materials used in molecular biology, cell culture, and mouse embryo manipulation. The one nonstandard piece of equipment is a cell-embryo fusion instrument to generate tetraploid embryos. This device is sold by BLS Ltd, Hungary (www.bls-ltd. com/fusion.html) under the name CF-150B. [Pg.171]

Eggan K, Jaenisch R (2003) Differentiation of FI embryonic stem cells into viable male and female mice by tetraploid embryo complementation. Methods Enzymol 365 25-39. [Pg.185]

Eggan K, Rode A, Jentsch I, Samuel C, Hennek T, Tintmp H, Zevnik B, Erwin J, Loring J, Jackson-Grusby L et al. (2002) Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation. Nat Bio-technol 20 455-459. [Pg.185]

Haploids can be produced from tetraploid cultivars and breeding clones via parthenogenesis (Hougas and Peloquin, 1957). When a tetraploid is crossed with any of several selected diploid clones, some of the offspring are diploid. In these crosses, both sperm cells from the pollinator enter the central cell, allowing normal endosperm to develop. This stimulates the division of the egg cell in the absence of fertilization, resulting in the production of a haploid (2x) embryo... [Pg.34]

Zhou T et al (2012) Generation of human induced pluripotent stem cells from urine samples. Nat Protoc 7(12) 2080-2089 Kang L et al (2009) iPS cells can support full-term development of tetraploid blastocyst-complemented embryos. Cell Stem Cell 5(2) 135-138... [Pg.348]

Fig. 2. ES cell-derived embryos by tetraploid complementation. RNAi transgenic ES cells (light gray) are injected into tetraploid host blastocysts (not shown) or aggregated with tetraploid morula embryos (dark gray). The chimeric embryos are allowed to develop in utCTo until the time of phenotypic analysis. The tetraploid cells contribute to the trophoblast portion of the placenta and the yolk sac endoderm. The knockdown ES cells develop into the entire embryo proper, as weU as yolk sac mesoderm and amnion (not shown). Fig. 2. ES cell-derived embryos by tetraploid complementation. RNAi transgenic ES cells (light gray) are injected into tetraploid host blastocysts (not shown) or aggregated with tetraploid morula embryos (dark gray). The chimeric embryos are allowed to develop in utCTo until the time of phenotypic analysis. The tetraploid cells contribute to the trophoblast portion of the placenta and the yolk sac endoderm. The knockdown ES cells develop into the entire embryo proper, as weU as yolk sac mesoderm and amnion (not shown).
Production of ES Cell-Derived Embryos by Tetraploid Complementation... [Pg.179]

Spawning, fertilisation, embryo development, early D-larvae and triploid and tetraploid induction... [Pg.344]

See other pages where Tetraploid embryo is mentioned: [Pg.317]    [Pg.339]    [Pg.115]    [Pg.179]    [Pg.183]    [Pg.142]    [Pg.909]    [Pg.317]    [Pg.339]    [Pg.115]    [Pg.179]    [Pg.183]    [Pg.142]    [Pg.909]    [Pg.39]    [Pg.169]    [Pg.169]    [Pg.41]    [Pg.573]    [Pg.14]   
See also in sourсe #XX -- [ Pg.339 ]



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