Terminal restriction fragment

Degradation of contaminants may occur with bacteria that have been isolated from pristine environments without established exposure to the contaminants, and exhibit no dependence on substrate concentration. For example, organisms from a previously unexposed forest soil were able to degrade 2,4,6-trichlorophenol at concentrations up to 5000 ppm, and terminal restriction fragment length polymorphism analysis revealed that at concentrations up to 500 ppm, the bacterial community was unaltered (Sanchez et al. 2004). 

FISH, terminal restriction-fragment length polymorphism analysis (rRNA-based molecular techniques) and comparative 16S rDNA analysis... 

T-RFLP Terminal restriction fragment length polymorphism... 

Liu WT, Marsh TL, Cheng H, Forney LJ (1997) Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl Environ Microbiol 63 4516 1522... 

Marsh, T. L. 1999. Terminal restriction fragment length polymorphism (T-RFLP) An emerging method for characterizing diversity among homologous populations of amplification products. Current Opinion in Microbiology 2 323-327. 

Osborn, A. M., Moore, E. R. B., and Timmis, K. N. (2000). An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics. Environ. Microbiol. 2,39-50. 

Singh, B. K., Nazaries, L., Munro, S Anderson, I. C and Campbell, C. D. (2006). Use of multiplex terminal restriction fragment length polymorphism for rapid and simultaneous analysis of different components of the soil microbial community. Appl. Environ. Microb. 72, 7278-7285. 

AOB communities were detected by terminal restriction fragment analysis of amoA genes (Bernhard et al., 2005). Nitrosospira-likc sequences were also identified as the seawater dominants in this environment. 

Leuders, T., and Friedrich, M. W. (2003). Evaluation of PGR amplification bias by terminal restriction fragment length polymorphism analysis of smaU-subunit rma and mcra genes by using defined template mixtures of methanogenic pure cultures and soil DNA extracts. Appl. Environ. Microbiol. 69, 320-326. 

Moeseneder, M. M., Arrieta, J. M., Muyzer, G., Winter, C., andHemdl, G.J. (1999). Optimization of terminal-restriction fragment length polymorphism analysis for complex marine bacterioplankton communities and comparison with denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 65, 3518-3525. 

Scala, D. J., and Kerkhof, L. J. (2000). Horizontal heterogeneity of denitrifying hacterial communities in marine sediments hy terminal restriction fragment length polymorphism analysis. Appl. Environ. Microbiol. 66, 1980—1986. 

Detail microbial study is crucial to correlate hydrogen production to the microbial group involved. For this purpose, a nucleic acid based technique - terminal restriction fragment length polymorphism (T-RFLP) - was used to identify the... 

Terminal Restriction Fragment Length Pol5miorphism Thermovolumetric analyzer University of Hawaii University of Illinois at Chicago United Technologies Research Center... 

Terminal restriction fragment length polymorphism (T-RELP) (Liu et al., 1997 Thies, 2007) is used for rapid analysis of microbial community diversity in various environments. The technique employs PCR in which one of the two primers used are fluorescently labeled and used to amplify a selected region of bacterial genes encoding 16S rRNA from total community DNA (Liu et al.,... 

Thies, J. E. 2007. Soil microbial community analysis using terminal restriction fragment length polymorphisms. Soil Sci. Soc. Am. J. 71 579-591. 

Klamer, M., Roberts, M.S., Levine, L.H., Drake, B.G., Garland, J.L., 2002. Influence of elevated CO2 on the fungal commuiuty in a coastal scrub oak forest sod investigated with terminal-restriction fragment length polymorphism analysis. Appl. Env. Microb. 68, 4370-4376. 

Terminal restriction fragment length polymorphism (TRFLP) uses end-labeled primers that will bind to specific primer sequences used when amplifying DNA by PCR [110,111]. After digestion of the PCR products with restriction enzymes, the samples are loaded into the CE system for separation. 

Clement, B., Kehl, L., DeBord, K., et al.. Terminal restriction fragment patterns (TRFPs), a rapid, PCR-based method for the comparison of complex bacterial communities, 7 Micro Wo/Mct/j, 31, 135, 1998. 

Dunbar, J., Ticknor, L., and Kuske, C., Assessment of microbial diversity on four southwestern United States soils by 16S rRNA gene terminal restriction fragment analysis, Appl Environ Microbiol, 66, 2943, 2000. 

Terminal Restriction Fragment Length Polymorphism (T-RFLP)... 

Marsh TL, Saxman P, Cole J, Tiedje J (2000) Terminal restriction fragment length polymorphism analysis program, a web-based research tool for microbial community analysis. Appl Environ Microbiol 66 3616-3620... 

---