Template molecule preparation

In-house experience with Cresset has shown that FieldPoint patterns can be particularly sensitive to conformation and charge. In the absence of a bioactive conformation for the template molecule, a suitable conformation and charge state 

These differences suggest how charge has a dramatic effect on the FieldPoint pattern around a molecule, and consequently, a field-based alignment of these molecules with one another would not be possible. It is clearly essential that the charge states of the template and database molecules match to ensure that correct alignments are obtained. In this experiment, the protonated form was used for the calculations, thus removing the potential mismatch of benzimidazole tautomers. 

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Zeolites are prepared by the linking of basic structural units around a template molecule. The structural units are typically based on oxides of silicon and aluminium, and the templates are usually individual small molecules. Under the right conditions, the silicon and aluminium oxide precursors will link up around the template to form a crystalline three-dimensional matrix containing the template molecules. The template... 

The pore size of most zeolites is <1.5nm. This microporosity limits their utility in most areas of chemistry, where the molecules used are much larger, and for which mesoporous materials would be necessary. Unfortunately, attempts to use larger template molecules in the zeolite synthesis, an approach which should in theory lead to larger pore size zeolites, have met with very little success. Indeed, some zeolitic materials have been prepared which have mesopores - none of these has ever displayed any real stability and most collapse on attempts to use them. A new methodology was thus required. 

Electrochemical template-controlled sjmthesis of metallic nanoparticles consists of two steps (i) preparation of template and (ii) electrochemical reduction of metals. The template is prepared as a nano structured insulating mono-layer with homogeneously distributed planar molecules. This is a crucial step in the whole technology. The insulating monolayer has to possess perfect insulating properties while the template has to provide electron transfer between electrode and solution. Probably, the mixed nano-structured monolayer consisting of alkylthiol with cavities which are stabilized by the spreader-bar approach [19] is the only known system which meets these requirements. 

The SSZ-33 zeolite was synthesized using the template molecule derived from 8-keto tricycle [5.2.1.0] decane. The boron containing SSZ-33 was converted to Al-SSZ-33 by a one-step reflux in 1 M aluminum nitrate. The SSZ-35 zeolite was prepared with the template camphorquinone. SSZ-35 zeolite was then subjected to three-fold ion exchange in ammonium nitrate. 

To prepare artificial enzymatic systems possessing molecular recognition ability for particular molecules, molecular imprinting methods that create template-shaped cavities with the memory of the template molecules in polymer matrices, have been developed [22, 30-35] and established in receptor, chromatographical separations, fine-chemical sensing, etc. in the past decade. The molecular... 

The uncalcined and unmodified sample is denoted as MCM-U. This uncalcined sample, i.e., sample with template molecules inside mesopores, was functionalized via one-step process that includes simultaneous trialkylsilanization and extraction of the template. Two modified samples were prepared with trimethylsilyl and octyldimethylsilyl groups attached to the mesopore walls and denoted as MCM-UM and MCM-UO, respectively. A typical synthesis [17] was carried out as follows about 0.2 g of the uncalcined MCM-41 was dispersed in 10 ml of trialkylchlorosilane, and refluxed for 36 hours (modification scheme is shown on Figure 1). Subsequently, about 5 ml of anhydrous pyridine was added to the mixture and refluxed for next 18 hours. After cooling down, the mixture was filtered and washed several times with small portions of ethyl alcohol, mixture of ethyl alcohol and... 

The P-turn-containing template molecule (see also Section 13.1.1.1.2) (0.352mmol) was dissolved in anhyd DMF (0.25 mL), TV-maleoy I - (5- a I ani n e V-hydroxysuccinimide ester (35 1.4 mmol, 4 equiv with respect to the e-amino groups of lysine residues) was added and the mixture was stirred at rt. The formation of the functionalized template molecule 36 was followed by analytical HPLC and was complete after 1 h. A soln of the thiopeptide 37 (1.408 mmol) in H20, pH 6-7, was added. The formation of the corresponding TASP molecule 38 occurred within 10 min. The mixture was lyophilized and the target TASP 38 was isolated as the major component by preparative HPLC yield 85%. 

A good imprinting effect also relies on the existence of stable assemblies between the template molecule and the functional monomers in the course of the polymerisation process therefore, any preparation method for beaded MIPs must preserve such template-monomer assemblies. 

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