TaqMan probes chemistry

Hydrolysis probes (e.g.,TaqMan probes) are the most widely used and pubhshed in detection chemistry hterature for probe-based real-time PCR apphcations. In addition to the primers, it includes a third oligonucleotide, 20 to 26 bases in length, in the reaction known as the probe. A fluorescent reporter dye, most frequendy... 

For studies of hematological malignancies, the sequence-specific fluorescence probe-based systems provide the advantage of an important further test for identification of the sequence of interest by virtue of hybridization of fluo-rescently labeled internal probes to the amplified target sequence. The most commonly used sequence-specific chemistries include the exonuclease (TaqMan), the linear hybridization probe, and the hairpin-based (Molecular Beacon) systems. (See Chapter 37 for further information on nucleic acid techniques.)... 

A similar technology relies on hydrolysis probes, namely, Taqman chemistry. Two probes are designed, one a perfect match to the normal allele and one to the mutant or variant allele. In Taqman chemistry, probes are released from quenching when the Taq polymerase degrades the probe, releasing the fluorophore into solution. This technology was developed from Applied Biosystems (Foster City, California) and is compatible with the 7900 real-time instrument (Applied Biosystems). Taqman chemistry also is developed easily by individual laboratories [7]. 

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