Tacrine analysis

Qizilbash N, Whitehead A, Higgins J, Wilcock G, Schneider L, Farlow M. (1998). Cholinesterase inhibition for Alzheimer disease a meta-analysis of the tacrine trials. Dementia Trialists ... 

Camps, R Contreras, J. Font-Bardia, M. Morral, J. Munoz-Torrero, D. Solans, X. Enantioselective synthesis of tacrine-huperzine A hybrids. Rreparative chiral MRLC separation of their racemic mixtures and absolute configuration assignments by x-ray diffraction analysis. Tetrahedron-Asymmetry, 1998, 9(5) 835-849. 

A, Sherwood J et al (2007) Tacrine-induced liver damage An analysis of 19 candidate genes. Pharmacogenet Genomics 17 1091-1100... 

Alhomida, A. S Al-Rajhi, A. A., Kama). M. A., and Al-Jafari, A. A. (2(XX)). Kinetic analysis of the toxicological etteci of tacrine (Cognex) on human retinal acetylcholinestera.se activity. ToxU oloity 147,33-39. 

The second study was conducted in patients with tacrine-induced liver injury which, unlike diclofenac-induced liver injury, does not appear to involve the adaptive immune system (Carr et al. 2007). Tacrine also produces transient and mild liver injury in rodents (Ma et al. 2003 Stachlewitz et al. 1997), and, as a result, preliminary experiments were able to identify genes associated with tacrine-induced liver injury in rats using gene chip analysis (Carr et al. 2007). These experiments revealed a significant increase in acute phase proteins in response to tacrine-induced liver injury (Carr et al. 2007). As IL-6 is a key regulator of the acute phase response, an attempt was made to correlate polymorphisms in the IL-6 gene with increased susceptibility to tacrine-induced liver... 

Holford, N. H. G. and Peace, K., The effect of tacrine and lecithen in Alzheimer s disease. A population pharmacodynamic analysis of five clinical trials, Eun. J. Clin. Pharmacol., 47 17-24, 1994. 

Tacrine and tluee metabolites (I-, 2-, and 4-hydroxytacrine) were extracted from plasma and separated on a Cig column (2 = 330 ran, ex 365 ran, em) using an 83/13 water (0.2 M acetate at pH 4.0)/acetonitrile mobile phase [1524]. Tacrine, the latest-eluting peak was detected at 35 min. Incomplete resolution of 1- and 2-hydroxytacrine occurred under these conditions. Almost 20 min of elution time occurred between the hydroxytacrine and tacrine elution. A gradient system would dramatically decrease analysis time and, if a weaker mobile phase is used initially, resolve the analytes mentioned above. Working standards ran from 6 to 240 nM and quantitation limits of 2-10 nM were reported (analyte dependent). 

Tacrine and seven metabolites (e.g., 2-hydroxy-, 4-hydroxy-, and 1-hydroxy-tacrine) wbre resolved on a phenyl colunm (photodiode array detector, X = 230-360 ran or UV at 2 = 325 ran) using a 70/30 acetonitrile/Avater (ammonium formate pH 3.1) mobile phase [1549]. The analysis required 25 min. Preparative LC was conducted with identical mobile phase conditions and a preparative-scale phenyl column. 

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