T7 phage

FIG. 1 Monolayer of T7 phage heads. Cryoelectron micrograph of tailless mutant of T7 bacteriophage. Bar 50 nm. (From Ref. 42. Copyright 1997 Cell Press.)... 

Danner, S. and Belasco, J. G. (2001) T7 phage display A novel genetic selection system for cloning RNA-binding protein from cDNA libraries. Proc. Natl. Acad. Sci. USA 98, 12,954-12,959. 

Viral mutation. An mRNA transcript of a T7 phage gene contains the base sequence... 

Fig. 11.2. Evaluation of RNAP and QD binding on the T7-phage genome, (a) Amplitude and (b) phase AFM scans of the T7-DNA-RNAP-QD complex. Protrusions of two typical sizes, corresponding to the RNAP and RNAP-QD are visible on the DNA backbone (Scale-bar 1pm). (c) Cross section along the DNA backbone depicting the height of the varions protrusions...

Fig. 11.3. Evaluation of stretching uniformity, (a) Histogram constructed from the end-to-end length of seventy T7-DNA-RNAP-QD complexes. A Gaussian fit to the data is centered at 11.75 xm corresponding to 86% of the contour length. The width of the distribution is 1.6 pm corresponding to about 12%. (b) Cropped fluorescence images of fuU length T7-phage genomes flow stretched on a polylysine coated coverslip...

Standard conditions are 20 hr at 45,000 rev/min using 1 to 5 /ig DNA. Densities are normally calculated with reference to a DNA of known density which is present in the centrifuge cell with the DNA to be analysed. Usually a standard DNA density 10 or 20 mg/ml above or below the sample DNA can be used (e.g. Micrococcus lysodeiktius, p= 1.731 g/ml Escherichia coli or T7 phage, p= 1.710 g/ml Sober 1968). The density is given by... 

Takakusagi, Y., Kobayashi, S., Sugawara, F. (2005). Camptothecin binds to a synthetic peptide identified by a T7 phage display screen. Bioorg. Med. Chem. Lett., 15, 4850-4853. 

Lane 1 marker DNAs G phage (630 kb), T2 phage (180 kb) and T7 phage (40 kb). Other lanes, yeast strains D273-10B/A1 loaded alternatively with DBY782. Note variation of chromosome sizes between strains. 

Both cyanobacterial thioredoxins were expressed in E. coli. One gram of bacteria yielded approximately 20 mg of thioredoxin. T-1 was able to restore the wild type phenotype in E. coli strains lacking thioredoxin except for the growth of T7 phage (2). T-2 was not effective in the... 

Infection of cells with DNA phage may lead to the production of a new, phage-spedfic RNA-P. (e.g. T7 phage RNA-P. is a si e-chtiin protein, M, 107,000), or the host RNA-P. may be modfied with the addition of new protein subunits encoded by the phage (e.g. T4 and k phage) in each case the resulting RNA-P. is specific for transcription of phage DNA. 

Buchholtz, F. Schneider, F. W. 1987. Computer Simulation of T3/T7 Phage Infection Using Lag Times, Biophys. Chem. 26, 171-179. 

It has been shown that the endosialidase is the only factor mediating Kl-specificity since the T7 phage lacking the endosialidase gene is unable to infect E. coli K1 strains. However, after enzymatic removal of the K1 capsule by endosialidase, the naked E. coli K1 strain can be infected by T7 [38]. Vice versa, the KIF phage is unable to infect E. coli strains lacking a polysialic acid capsule [108]. 

Tobacco necrosis virus (TNV) general 77, 3 translation 11,4 Tobacco rattle virus (TRV) assembly 6, 2, 4, general 77, 1 translation 77, 4 T-odd (T3, T5, T7) phages general 7, 3 regulation 8, 1 Togaviridae... 

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