Chromosome sizes

Schwartz, D. C., and C. R. Cantor, Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37 67-75, 1984. 

The apparatus shown in Figure 27-18 is one type of a commercial design for vacuum blotting. The manufacturer says it is "Designed to handle all types of nucleic acid separations, the precision controlled vacuum pump maintains the exact vacuum level needed to blot all lengths of nucleic acids, from depurinated RNA or DNA fragments to chromosome size. No wicks, cassettes or stacks of filter paper are needed. It can do up to 10 transfers a day with close to 100% DNA recovery."... 

Lane 1 marker DNAs G phage (630 kb), T2 phage (180 kb) and T7 phage (40 kb). Other lanes, yeast strains D273-10B/A1 loaded alternatively with DBY782. Note variation of chromosome sizes between strains. 

Protocol for the Preparation of Chromosome-Size DNA for PFGE and Separation of Chromosomes by CHEF... 

Table 8.7 Karyotypes of 22 M. mucilagina strains (from Table 8.6). Chromosome size from 650 to 2300. Number corresponds to the column in Table 8.6. N haploid chromosome number. Host type abbreviations O Opuntia, C Ferrocactus and S Stenocereinae.

Chihuahuan Desert), F Florida and A Australia. Genome sizes are sums of chromosomal sizes. Chromosomes are marked with an X corresponding to the molecular weight of the hand. 

For integration of precedence-constrained production sequencing and scheduling, the authors [19-21] proposed a GA with variability of chromosome size to optimise the class of problems for one as well as two objective functions in one as well as multiple production line environments. 

Rothfels, K., E. Sexsmith, M. Heimburger, and M. O. Krause. 1966. Chromosome size and DNA content of species of Anemone L and related genera (Ranunculaceae), Chromosoma, 20 54-74. 

Chromosome velocity during mitosis as a function of chromosome size and... 

The key step in analyzing HMW DNA is to purify intact, chromosome-size molecules. Standard methods for extracting DNA from cells or organisms (e.g., phenol extraction and ethanol precipitation) produce fragments with an average size of 50-200 kb under... 

---