T7 bacteriophages

FIG. 1 Monolayer of T7 phage heads. Cryoelectron micrograph of tailless mutant of T7 bacteriophage. Bar 50 nm. (From Ref. 42. Copyright 1997 Cell Press.)... 

Figure 5.8. Schematic illustration of T7 bacteriophage display system. The gene encoding the protein of interest is fused to the gene 10 protein that encodes a coat protein in the head of the phage. Cloning is to the C-terminus of coat protein 10. Figure adapted from Sche et al. (1999).

Bacteriophage T7 Bacteriophage T7 and its close relative T3 are relatively small DNA viruses that infect Escherichia coli. (Some strains of Shigella and Pasteurella are also hosts for phage T7.) The virus particle has an icosahedral head and a very small tail. The virus particle is fairly complex, with S different proteins in the head and 3-6 different proteins in the tail. One tail protein, the tail fiber protein, is the means by which the virus particle attaches to the bacterial cell surface. Only female cells of Escherichia coli can be infected with T7 male cells can be infected but the multiplication process is terminated during the latent period. 

The role of OJ in strand breakage was shown with the DNA of the T7 bacteriophage, in this process OH radicals were implicated The possible relationship with spontaneous cancer has been stressed... 

Sheared and melted segments of bacteriophage T2 DNA (MW= 1.3X10 ) rena-tured 19.2% as fast as sheared and melted DNA from T7 bacteriophage under the same conditions of temperature, ionic strength, and concentration. What is the MW of T7 DNA ... 

Fig. 8.5 High-throughput screening at 10mMforWBZ 4 (red) andimatinib (STI 571, blue, control) over a battery of human kinases displayed in a T7-bacteriophage library (Ambit Biosciences, San Diego, CA). Hit values are reported as percentage-bound kinase. Reprinted from the [14], copyright 2007 with permission from the American Society for Clinical Investigation...

As shown in Chap. 8, WBZ 4 is a re-engineered version of imatinib with an enhanced specificity needed to curb imatinib s potential cardiotoxicity. The inhibitory impact of WBZ 4 is restricted to KIT, PDGFR, and JNK, all with nanomolar affinity, as corroborated by screening against a T7-bacteriophage library of kinase displays (Ambit Biosciences, CA) [13],... 

The exogenous RNA template (350 bp) used in the Amp-RT assay is prepared from a plasmid-cloned sequence of the encephalomyocarditis virus (EMCV) (positions 7114-7516, GenBank accession number M81861), and is generated by in vitro transcription of a T7-tagged PCR product by using T7 bacteriophage RNA polymerase. 

Note Results [83H1] of total intensity light scattering on linear T7 bacteriophage DNA (M=26.43-10 g-mol , from sequence analysis), analyzed according to Sharp and Bloomfield [68S1],... 

Fig. 1. Growth curve of T7 bacteriophage after multiple infection of P Mabeled bacteria in nutrient broth. , light transmission of the whole culture at 660 m , phage titer o, radioactivity of the supernatant solution after centrifugation of aliquots of the infected cells. Note that the physical methods give the same stepwise curve as does biological assay (262).

Figure 11.5 Modeling of packaging of dsDNA into T7 bacteriophage. (From Forrey, C. and Muthukumar, M., Biophys.J., 91, 25, 2006. With permission.)...

Herrlich, P., Schweiger, M. T3 and T7 Bacteriophage deoxyribonucleic acid-directed enzyme synthesis in vitro. J. Virol. 6, 750-753 (1970). 

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