Synthetic peptides formation

One glycosylation site exists on the P suburhts of human LH [53664-53-2 and TSH [64365-92-OJ ie, Asn-30 (Fig. 4). In some species, Asn-13 of LH-P is glycosylated (48). FSH-P suburnt [58857-12-8] is glycosylated at two sites, Asn-13 and 30. Based on interactions of synthetic peptides with the LH receptor, loops formed by P93—100 and P38—57 may be essential for hormone bioactivity (48). Highly conserved sequences between residues 31—37 have been implicated in the formation of the a—P suburnt dimer (48), which is absolutely necessary for the expression of bioactivity. 

Chabry,J., Caughey, B., and Chesebro, B. (1998). Specific inhibition of in vitro formation of protease-resistant prion protein by synthetic peptides. /. Biol. Chem. 273, 13203-13207. 

Castano, E. M., Ghiso, J., Prelli, F., Gorevic, P. D., Migheli, A., and Frangione, B. (1986). In vitro formation of amyloid fibrils from two synthetic peptides of different lengths homologous to Alzheimer s disease beta-protein. Biochan. Biophys. Res. Commun. 141, 782-789. 

The binding of integrins to certain ligands is mediated by a short amino acid sequence. Thus, the binding site for fibronectin is confined to a sequence of four amino acid residues, Arg-Gly-Asp-Ser (RGDS) (R7). Synthetic peptides containing the RGDS sequence prevented adhesion of cells to fibronectin (B4, R7) and also inhibited metastasis formation in animal models (B4, R7). 

This designed construct, as such, may not influence subsequent steps of transamination due to loss of the strong association between the coenzyme and the synthetic peptide after amino acid binding. However, the selectivity of the peptide for pyridoxal phosphate reveals the potential power of peptide design and the importance of secondary binding interactions for defining the formation of specific binary complexes. 

Spin-labelled synthetic peptides were used to study coil-coil formation.41 Strong exchange interaction at sites near the N-terminus of a 35-amino acid peptide demonstrate parallel coil formation. In a 30-residue peptide corresponding to residues 468-497 of osmoregulatory transporter ProP, strong spin-spin interaction was observed for label near the centre of the peptide and weak interaction was observed for label near the C-terminus, consistent with antiparallel coil alignment. 

Hoffman MP, Nomizu M, Roque E et al. Laminin-1 and laminin-2 G-domain synthetic peptides bind syndecan-1 and are involved in acinar formation of a human submandibular gland cell line. J Biol Chem 1998 273 28633. 

Active immunization occurs if the antibodies formed protect the organism from viruses or toxins. It is hoped that synthetic peptides triggering the formation of antibodies will find use as potential vaccines. 

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