Synthesis of Polynucleotides on Polymer Supports

Type of linkage Phase Products Yield (%) References 

Poly-(L-Lys)—NHC—CeH4—NHj Glass beads-Si-TrT Sephadex-(2-ethoxyethyl)-pU Poly(ethylene glycol)-0-MeOTr-T 

Poly( ethylene)—S—C6H4—CHsO—pT ( )—C6H4—C0(CH2)2C02—rU 

Yip and Tsou419 initiated research in this area, with a synthesis of UpUpU on an insoluble, isotactic, succinylated polystyrene the yields were 50% in each coupling step. 2 -0-(2-Benzoylpropanoyl)-3 -0-(methoxyacetyl)uridine was linked to the support, and mild treatment with alkali enabled 2, 3 -0-protected uridine 5 -phosphate to be added similarly. The dinucleotide CpUp was synthesized420 by attach- 

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