Syntheses of Polynucleotides

The ditetrazolide of methylphosphite plays a role in the solid phase synthesis of polynucleotides. The ditetrazolide, which is stable for weeks when stored at —20 °C, is prepared in a simple condensation reaction between methylphosphordichloridite and two equivalents of tetrazole in anhydrous tetrahydrofuran in the presence of 2,4,6-tri-methylpyridine.t28]... 

Tetrazolide-activated nucleotide phosphites have been employed in the synthesis of polynucleotides on a polymer support using methoxybistetrazolylphosphine as phosphi-tylating agent [104]... 

Fig. 1. Light-directed synthesis of polynucleotide probe arrays. Exposure to light through a photolithographic mask is used to remove protecting groups from surface sites in pre-defined regions of a functionalized glass substrate. A solution of an activated polynucleotide building block is then applied, which reacts specifically in the exposed regions of the substrate. Repeated cycles of illumination-deprotection and monomer coupling are used to construct a two-dimensional array of probe sequences...

Table 1. Combinatorial synthesis of polynucleotide probe arrays...

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