Synaptosomal membrane ratio

By feeding nutritionally adequate diets, dietary intake of 18 2n-6, 18 3n-3, or the proportion of 18 2n-6 to 18 3n-3, particularly during development, has been shown to influence the content of long-chain polyunsaturated fatty acids in membrane lipids by changing the composition of the whole brain, oligodendrocytes, myelin, astrocytes mitochondrial, microsomal, and synaptosomal membrane (Bourre et al., 1984 Foot et al., 1982 Lamptey Walker, 1976 Tahin et al., 1981). Feeding diets with a 18 2n-6 to 18 3n-3 fatty acid ratio between 4 1 and 7 1 to rats from birth to 1, 2, 3, and 6 wk of... 

Preliminary experiments to delineate the optimum conditions for [ H]MDA and [ HJMDMA binding to rat brain membranes indicated that the best signal-to-noise ratio was found using a crude synaptosomal preparation incubated at 4 °C. While most experiments employed 50 mM Tris-HCl (pH 7.1) as the incubation medium, we found that the addition of 0.27 M sucrose to the incubation medium increased the specific binding approximately fivefold. Subsequent experiments were carried out under both conditions. Other preliminary experiments suggested that [ H]MDA and... 

Studies on the sodiiun stimulated 5-HT uptake in cerebral synaptosomes as for example by Wood using synaptosomes of rat cerebral cortex [20] or by Mann and Hrdina using synaptosomes of rat diencephedon [21] suggested a 5-HT" Na = 1 1 ratio similar to that found using porcine platelet membranes. 

---