Membrane synaptosomal

Krueger, B.K. Ratzlaff, R.W. Strichartz, G.R. and Blaustein, M.P. Saxitoxin binding to synaptosomes, membranes and solubilized binding sites from rat brain. J. Membr Biol 50 287-310,... 

The initial step after cellular uptake of T4 is metabolic transformation to 3,5,3, -tri-iodothyronine (T3) (Fig. 52-8), which interacts with cytosolic and nuclear receptors, as well as with synaptosomal membrane binding sites of unknown function [25], Cytosolic receptors are proteins of 70 kDa that do not appear to undergo translocation to cell nuclei, nor do they appear to be nuclear proteins that have leaked out of cell nuclei during cell rupture nuclear receptors are proteins of 50 70 kDa that have both DNA-and hormone-binding domains [25,26,28],... 

Bondy SC, McKee M, Davoodbhoy YM. 1990a. Prevention of chemically induced changes in synaptosomal membrane order by ganglioside GMI and alpha-tocopherol. Biochim Biophys Acta... 

Evans, D., Williams, R.S., Shone, C.C., Hambleton, P., Melling, J. and Dolly, J.O., Botulinum neurotoxin type B. Its purification, radioiodination and interaction with rat-brain synaptosomal membranes, Eur. I. Biochem., 154, 409-416, 1986. 

Edelfors S, Raven-Jonsen A. 1992. Effect of organic solvents on nervous cell membrane as measured by changes in the calcium magnesium ATPase activity and fluidity of synaptosomal membrane. Pharmacology and Toxicology 70(3) 181-187. 

Anandamide inhibited the specific binding of [ H]-HU-243 to synaptosomal membranes in a manner typical of competitive ligands, with an inhibition constant (K ) of 39.0 + 5.0 nM. In this system, the of tsP-THC, a psychoactive compound of cannabis, was 46.0 + 3-0 nM. These were exciting results — the psychoactive compound from a higher plant and a chemically completely different compound in the brain were found to bind to the same brain receptor at about the same level of activity. Soon after the identification of anandamide, this compound was tested for its pharmacological activity. Anandamide administered i.p. in mice, caused... 

Gangliosides. sialidase and sialvltransferase in the membranes surrounding nerve endings ( synaptosomal membranes )... 

Figure 5. Electron microscopic examination of the "nerve ending fraction (X13,000) and of the synaptosomal membrane fraction (X.7150)...

Parameter Nerve ending fraction Activity (or concentration) total specific Synaptosomal membrane fraction Activity (or concentration) total specific Enrichment ... 

Figure 6. Effect of CMP-NeuAc concentration (V/S), of pH (V/pH), of enzymatic protein concentration (V/protein), and of incubation time (V/t) on the activity of synaptosomal membrane-bound sialyltransferase. Calf brain cortex. Acceptor substrates for sialyltransf erase (if) lactosylceramide ( ) desialylated fetuin (%) endogenous glycoprotein (endogenous glycolipids.

An experimental model for the study of ganglioside behavior in synaptosomal membranes... 

Tzeng MC, Siekevitz P (1979) The binding interaction between a-latrotoxin from black widow spider venom and a dog cerebral cortex synaptosomal membrane preparation. J Neurochem... 

Gotow T, Miyaguchi K, Hashimoto PH (1991) Cytoplasmic architecture of the axon terminal filamentous strands specifically associated with synaptic vesicles. Neuroscience 40 587-98 Gower H, Rodnight R, Brammer MJ (1986) Ca2+ sensitivity of Ca2+-dependent protein kinase activities toward intrinsic proteins in synaptosomal membrane fragments from rat cerebral tissue. J Neurochem 46 440-7... 

Another example is the perturbing effect of eight calcium channel blockers on membranes prepared from two different lipids [68]. The authors used total lipids from rat brain and synaptosomal membranes. The spin probe was l-palmitoyl-2-stearoyl-phosphatidyl-choline labeled at the doxyl group at the carbon-16 position (16-PC). The apparent order parameter, S, is calculated from the apparent outer (Amax) and inner (Amin) splittings which were directly taken from the ESR spectra. It is used to describe the relative efficiency of the dmgs in perturbing the lipid membrane. 

Changes in EFA status affect the activity of several membrane-associated enzymes and proteins 68-71 Reduced adenyl cyclase activity occurred in EFA-deficient animals,72 while in animals supplemented with n-6 or n-3 fatty acids, increased adenyl cyclase activity was seen in cardiac membranes.73,74 However, the opposite effect has been reported in other membranes, possibly reflecting differences in initial fatty acid composition.75 n-3 PUFAs have been shown to activate membrane Ca-ATPase and inhibit Na, K-ATPase in isolated basolateral membranes from rat duodenal enterocytes76 and inhibit both Ca-ATPase and Na,K-ATPase activity in synaptosomal membranes isolated from rat cerebral cortex.77 EFAs have the ability to modify neuronal Ca-ATPase activity... 

Figure 7.21. Homeoviscous adaptation of brain synaptosomal membranes. (A) Effects of measurement temperature on the physical states of synaptosomal membranes of differently adapted vertebrates, as determined by fluorescence anisotropy using l,6-diphenyl-l,3,5-hexatriene (DPH) as the probe. (B) DPH anisotropy at each species adaptation temperature. The horizontal lines enclose species where essentially complete homeoviscous adaptation was found. (Figure modified after Logue et al., 2000.)...

Javitt DC, Balia A, Sershen H. 2002. A novel alanine-insensitive D-serine transporter in rat brain synaptosomal membranes. Brain Res 941 146-149. 

H17. Hensley, K., Carney, J., Hall, N., Shaw, W., and Butterfield, D. A., Electron paramagnetic resonance investigations of free radical-induced alterations in neocortical synaptosomal membrane protein infrastructure. Free Radicals Biol. Med. 17, 321-331 (1994). 

Ondrias, K., Stasko, A., Marko, V., and Nosal, R. (1989), Influence of beta-adrenoceptor blocking drugs on lipid-protein interaction in synaptosomal membranes. An ESR study, Chem. Biol. Interact., 69, 87-97. 

The aminopeptidase was obtained from either serum, rat brain, or synaptosomal membrane. 

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