Surface enhanced laser desorption ionisation

Merchant M, Weinberge S (2000) Recent advancements in surface enhanced laser desorption/ ionisation time of flight mass spectrometry. Electrophoresis 21 1164—1167 Petricoin EF, Ardekani AM, Hitt BA, Levine PJ, Fusaro VA, Steinberg SM, Mills GB, Simone C, Fishman DA, Kohn EC and Liotta LA (2002) Use of proteomic patterns in serum to identify ovarian cancer. Lancet 359 572-577 Rabilloud T (2000) Proteome Research two dimensional gel electrophoresis and identification methods. Springer Ver-lag, Berlin Heidelberg... 

Surface enhanced laser desorption ionisation (SELDI) is a version of MALDI with the addition of a special chip from which sample components are ionised and desorbed. The chip can be a protein chip array composed of spots of different chromatographic surfaces (which are chemical or biochemical in nature) designed to retain specific proteins. Its main advantage is inclusion of this separation/selection step for crude or complex samples prior to MS analysis. 

SELDI surface enhanced laser desorption ionisation... 

In non-laser desorption/laser ionisation techniques selective laser ionisation of gas phase neutrals (atoms and molecules), generated by evaporation, thermal heating, particle or photon bombardment, can be applied. Regarding ionisation, it is possible to differentiate between thermal impact with other particles (LEIS laser-enhanced ionisation), ionisation due to an electric field (FILS field ionisation laser spectrometry) and that due to photoionisation (RIS resonance ionisation spectrometry). This type of ionisation can be combined with a mass spectrometer to detect isotopes selectively (RIMS resonance ionisation mass spectrometry). If a polymer additive is volatile enough its presence can be ascertained via ionisation-only spectra. That is, the additive evaporates from the near-surface region for a certain amount of time, and the ionisation laser alone will yield a reasonable amount of signal to obtain an analysis of the additive present. 

Laser desorption/Iaser post-ionisation (two-shot LDI) can display either non-specific detection (using non-resonant single-photon ionisation) or species-specific detection (using resonance-enhanced multiphoton ionisation). By exercising both of these options, complicated mixtures can be analysed for surface species. As efficiency of mass spectral analysis is greatly enhanced by ionising the... 

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