Surface Display Technologies

Ligand binding, and certain enzymatic assays, can be greatly simplified if a protein is biosynthetically anchored on the external surface of a cell. Surface displayed proteins are readily accessible to fluorescent probes and thus, transport limitations are circumvented. In addition, 

Finally, protein display on the surface of insect cells has also been described (Ernst et al., 1998). Proteins expressed in insect cells are more likely to be properly glycosylated and correctly folded, relative to microbial hosts. However, constructing protein libraries of significant diversity in insect cells is likely to be a challenge. 

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This section will describe a robust method for engineering a binding protein of interest using yeast surface display technology. Similar methods for gene engineering via yeast display have been reported (9, 31, 32), and slight variations may be used for particular applications to attain optimal results. 

One disadvantage of both phage and yeast surface display-technologies Hes in the fact that the displayed peptide or protein is rather small in size compared to... 

Triarylamine is a purely organic molecule which is interesting as a chro-mophore in e.g. display technology. The molecule can be switched between a reduced colourless, and an oxidized blue state. The sensitization to nanos-tructured TiC>2 electrodes provides the substantial surface area required for a strong coloration. It is, however, believed that the electron transfer involved in the redox reaction takes place mainly within the sensitizer layer, and does not involve the substrate. Instead, there is an eventual electrical contact between the back-contact and the sensitizer layer [98]. For a quantum chemical modelling of the system, the inclusion of the substrate is in this case not likely to be essential. For a molecule of this size, it is possible to apply standard quantum... 

Phage display technology enables in vitro selection from libraries of proteins or peptides displayed on the surface of filamentous bacteriophages. It was introduced by George P. Smith in 1985 [2] and, since then, several thousands research papers... 

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