Superoxide dismutase purification

A10. Asayama, K., and Burr, I. M., Rat superoxide dismutases—Purification, labeling, immunoassay, and tissue concentration. J. Biol. Chem. 260, 2212-2217 (1985). 

Rl. Reiss, U., and Gershon, D., Rat liver superoxide dismutase Purification and age-related modification. Biochem. Biophys. Res. Commun. 73, 255-262 (1976). 

Water soluble protein with a relative molecular mass of ca. 32600, which particularly contains copper and zinc bound like chelate (ca. 4 gram atoms) and has superoxide-dismutase-activity. It is isolated from bovine liver or from hemolyzed, plasma free erythrocytes obtained from bovine blood. Purification by manyfold fractionated precipitation and solvolyse methods and definitive separation of the residual foreign proteins by denaturizing heating of the orgotein concentrate in buffer solution to ca. 65-70 C and gel filtration and/or dialysis. 

Dos Santos WG, Pacheco I, Liu MY, et al. 2000. Purification and characterization of an iron superoxide dismutase and a catalase from the sulfate-reducing bacterium Desulfovibrio gigas. J Bacteriol 182 796-804. 

Lehmann Y, Meile L, Teuber M. 1996. Rubrerythrin from Clostridium perfringens cloning of the gene, purification of the protein, and characterization of its superoxide dismutase function. J Bacteriol 178 7152-8. 

Isolation, Purification, Characterization, and Assay of Antioxy-genic Enzymes Isolation and characterization of superoxide dis-mutase, 105, 88 superoxide dismutase assays, 105, 93 assays of glutathione peroxidase, 105, 114 catalase in vitro, 105, 121 assays of lipoxygenase, 105, 126. 

Superoxide dismutase Bovine red blood cells AOT/heptane Extraction and purification [68]... 

Cermenati L, Pichat P, Guillard C, Albini A. Probing the Ti02 photocatalytic mechanisms in water purification by use of quinoline, photo-Fenton generated OH radicals and superoxide dismutase. J Phys Chem B 1997 101 2650-2658. 

M27. Miyata-Asano, M., Ito, K., Ikeda, H., and Sekiguchi, S., Purification of copper-zinc-superoxide dismutase and catalase from human erythrocytes by copper-chelate affinity chromatography. J. Chromatogr. 370, 501-507 (1986). 

W8. Weselake, R. J., Chesney, S. L., Petkau, A., and Friese, A. D., Purification of human copper,zinc superoxide dismutase by copper chelate affinity chromatography. Anal. Biochem. 155, 193-197 (1986). 

Le Trang, N., Meshnick, S. R., Kitchener, K., Eaton, J. W. and Cerami, A. (1983) Iron-containing superoxide dismutase from Crithidia fasciculata. Purification, characterization, and similarity to leishmanial and trypanosomal enzymes. J. Biol. Chem. 258 125-130. 

Rhoads, M. L. (1983) Trichinella spiralis identification and purification of superoxide dismutase. Exp. Parasitol. 56 41-45. 

When isolating superoxide dismutases from other tissues than erythrocytes the methods explained here are normally not suitable. For the purification from solid sources like liver a lot of other techniques are described Moreover, Mn-SOD and Fe-SOD are neither resistant to organic solvents nor to heat treatment. Therefore, mild conditions are required for the isolation of these enzymes. The techniques used... 

Most of the current work on manganese superoxide dismutases is limited on purification studies of these unique class of enzymes Recently the joint puri-... 

Dixit, D. Pushpangadan, R Kochhar, V. K. Kochhar, S. Rao, C. V. Purification and characterization of autoclavable and microwaveable superoxide dismutase from Curcuma longa and use in cosmetie, and pharmaceutical compositions. PCX Int. Appl. WO 2005017134, 2005 Chem. Abstr. 2005, 142, 256737. 

Table 7.5. Isolation and purification of superoxide dismutase from P. globosum Purification stq) Total activity. Total protein. Specific...

Giannopolitis CN, Ries SK (1977) Superoxide dismutases n. Purification and quantitative relationship with water-soluble protein in seedlings. Plant Physiol 59 315-318... 

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