Preparative supercritical fluid chromatography

Klesper, E. Corwin, A. H. Turner, D. A. J. Org. Chem., 1962, 27, 700. Sugiyama, K. Saito, M. Hondo, T. Seda, M. J. Chromatogr. 1985, 332, 107. Anderson, I. G. M. Supercritical fluid extraction coupled to packed column supercritical fluid chromatography. In Supercritical Fluid Extraction and Its Use in Chromatographic Sample Preparation Westwood, S. A. Ed., Blackie Academic and Professional, Chapman and Hall UK, 1993 p. 112. 

Packed-column SFC also is suitable for preparative-scale enatioseparations. Compared with preparative LC, sub- or supercritical fluid chromatography results in easier product and solvent recovery, reduced solvent waste and cost, and higher output per unit time. Because of its reduced sample capacity, SFC usually allows the separation of 10-100 mg samples per run. Chromatographers can compensate for these sample amounts by using shorter analysis times and repetitive injections (Wolf and Pirkle, 1997). 

Villeneuve, M.S., and Miller, L.A. (2005) Preparative-scale supercritical fluid chromatography, in Preparative Enantioselective Chromatography, 1st edn (ed. G.B. Cox), Wiley-Blackwell,... 

K. Anton, M. Bach and A. Geiser, Supercritical fluid chromatography in the routine stability control of antipruritic preparations , J. Chromatogr. 553 71 -79 (1991). 

Steuer et al. compared supercritical fluid chromatography with capillary zone electrophoresis (CZE) and high-performance liquid chromatography (HPLC) for its application in pharmaceutical analysis [24]. Efficiency, performance, sensitivity, optimization, sample preparation, ease of method development, technical capabilities, and orthogonality of the information were the parameters studied. They concluded that SFC is ideal for moderately polar compounds, such as excipients, for which mass detection is required. 

S. Scalia and D.E. Games, Determination of free bile acids in pharmaceutical preparation by packed column supercritical fluid chromatography, J. Pharm. Sci., 82 44 (1993). 

Highly stereoregular PMMA macromonomers, 14, prepared by Hatada and coworkers, have recently been fractionated by supercritical fluid chromatography into completely uniform fractions with no structural distribution [20,21]. They have been oligomerized with a radical (AIBN) or an anionic initiator (3,3-dimethyl-1,1-diphenylbutyllithium). After a new fractionation by SEC comb or star polymers of completely uniform architecture are obtained. No doubt, these samples will be most promising to investigate the branched structure-property relationship. 

Jusforgues, P. and Shaimi, M. 1998. Preparative supercritical fluid chromatography. 

Chiral Stationary Phases for GC and HPLC. Enantiomerically pure NEA has been used to prepare a variety of chiral stationary phases for liquid, gas, and supercritical fluid chromatography. These stationary phases are used to separate enantiomers without derivatization of the substrate with a chiral agent. 

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