Sunflower high oleic mutant

Fem dez-Martinez, J.M., Mancha, M., Osorio, J., and Garces, R. (1996) Isolation of an X-rays induced sunflower mutant containing high levels of palmitic acid in a high oleic background. Submitted. 

Blockage of A12-desaturase activity would be expected to result in the accumulation of oleic acid. Thus high oleic (acid) oils are available with mutant lines of soybean (Martin and Rinne, 1986), sunflower (Garces and Mancha, 1989 Sperling et al., 1990) and peanut (Powell et al., 1990). The relevant gene in sunflower is repressed in seed but not other tissues (Sperling et al., 1990)... 

Developing sunflower seeds of normal genotype and "high oleic acid" mutant (90% oleic acid, 1% linoleic acid) (1) have been used to study the incorporation of acetate into neutral and polar... 

BIOCHEMICAL CHARACTERIZATION OF A HIGH OLEIC ACID MUTANT FROM SUNFLOWER... 

The lipids of normal and a high oleic acid mutant (1) sunflower seeds have been biochemically characterized. "In vivo" and "in vitro" incubations with [l- c3 oleate showed a lack of oleate desaturase activity in developing mutant seeds. 

The aim of the present work was to study whether phopholipids, fatty acids, free sterols and membrane fluidity modulate vanadate-sensitive H -ATPase and vanadate-sensitive ATP-dependent transport activities in microsomes from etiolated sunflower cotyledons of a normal genotype and a mutant with high oleic acid content [5] during seedling establishment. 

Sunflower Helianthus artnuus) Tradilional line CAS-6 High stearic acid mutant CAS-14 Controlled temperature conditions GC experiments Day/night temperatures 20/ 10°C 25/15°C 30/20°C 35/25°C Entire cultivation cycle In CAS 6, oleic acid content increased with 34 points (fiom 22.7-59.6%) with an increase in the temperature In the high stearic acid mutant, CAS-14, oleic acid content increased with 10 points (from 26.2-36.5%) with an increase in temperature however, oleic acid content was reduced to 24.1% in the mutant at 35/25°C temperature range Eemandez-Moya et al. (2002)... 

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