Sulfation linkage region

Again, the product was identical to that of the naturally occurring trisaccharide unit in the chondroitin sulfate linkage region. 

In dermatan sulfate from shark skin, there is about a 40% excess of sulfate groups over 2-acetamido-2-deoxy-D-galactose residues.300 The small proportions of D-glucu-ronic acid (d-GUA) present in dermatan sulfate occur in regions of the carbohydrate chains both near to, and far from, the carbohydrate-peptide linkage region. The isolation of the tetrasaccharide... 

Evidence for the structure of the main repeating-chain of chondroitin sulfate, as well as the sequence at the linkage region to protein, has come... 

Two types of keratan sulfate, corneal and skeletal, have been differentiated by the hydrolytic behavior of the linkage region. The former has an N-... 

Oligosaccharide Fragments Isolated from the Carbohydrate-Protein Linkage-Region of Dermatan Sulfate Proteoglycans 1... 

Figure 2. Structures of the glycosaminoglycans and their linkage regions to protein. Two disaccharide repeating units are shown to emphasize the microheterogeneity that exists in some cases. Heparan sulfate and heparin show many structural similarities. However, heparan sulfate contains more GlcNAc(a-1 — 4)GlcUA repeating units fewer glucosamine residues are N-sulfated, and few iduronic acid residues are sulfated at C2.

Yamada S, Oyama M, Yuki Y, Kato K, Sugahara K. The uniform galactose 4-sulfate structure in the carbohydrate-protein linkage region of human urinary trypsin inhibitor. Eur J Biochem 1995 233 687-693. 

Sugahara K, Yamashina 1, Dewaard P, Vanhalbeek H, Vhegen-thart JFG. Structural studies on sulfated glycopeptides from the carbohydrate-protein linkage region of chondroitin 4-sulfate proteoglycans of swarm rat chondrosarcoma - demonstration of 86. the structure Gal(4-0-Sulfate)-P-l-3-Gal-P-l-4Xyl-P-l-0-Ser. J. 

Rio, S, Beau, J-M, Jacquinet, J-C, Synthesis of sulfated and phosphorylated glycopeptides from the carbohydrate-protein linkage region of proteoglycans, Carbohydr. Res., 255, 103-124, 1994. 

The proof of this sequence, and of the nature of the carbohydrate-peptide bond, was based on enzymic erosion to a glycopeptide fragment that was then studied by adaptations of classical methods. A similar linkage-region probably occurs in heparin,86 heparitin sulfate,87 dermatan sulfate88 and chondroitin 6-sulfate.88a It is possible that the polypeptide core itself is branched.89... 

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