Sugars enzymes

PG activity was assayed from cells grown in a medium containing 1% glucose in one-litre self-induced anaerobic fermentation for 5 days by increase of reducing sugars. Enzyme activity increased from pH 3.0 to pH 5.0 (citrate buffer) and decreased drastically above 5.0 (phosphate buffer), but activity was not affected differentially by the two buffers used (data not shown). PG activity increased almost linearly from 20°C to 40°C but above this optimum, activity was lost rapidly and the enzyme was completely inactivated at 60°C and 70°C after 10 and 6 min, respectively (data not shown). No PL, PGL or PME were detected. 

Arthur Harden Great Britain fermentation of sugar and sugar enzymes... 

Nucleotide sugar Enzyme/EC number Enzyme source Nat. Rec. In situ Reg. Synthesized structure References... 

Sucrose. See also, Sugar, enzymic synthesis of, 6, 29-48 structure and configuration of, 4, 1-35 utilization of, 4, 293-336... 

CWR by Additives, in which Tween 20 was added before the addition of en2ymes. During hydrolysis, 1-ml aliquots were taken at start and after 1, 2, 4, 8, 24, 48, and 72 h of hydrolysis. The sugars, enzyme protein concentration, and activity were measured. 

The specificity of enzymes arises in the way enzymes bind their substrates. An ct-glycosidase will only bind the a stereoisomeric form of a glycoside, not the j8 form. Enzymes that metabolize sugars bind only D sugars enzymes that synthesize most proteins bind only l amino acids and so on. 

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