Sucrose polysaccharide synthesis

A fructan-produclng bacterium was Isolated from soils and characterized for polysaccharide synthesis. The composition and properties of the polysaccharide produced were studied. The organism. Identified as a strain of Bacillus polvmvxa. produced a large quantity of polysaccharide when grown on sucrose. 

Oligo- and Polysaccharide Synthesis with Sucrose Analogues... 

All these oligo- and polysaccharides in nature are synthesized in vivo) via sucrose. Technical synthesis (in vitro) found industrial practices mainly for oligofructose, Neo-amylose and dextran following the reaction pathways as outlined in Figure 9.3.7. 

The marked advances made in the study of bacterial polysaccharides serve only to emphasize the vast amount of work still to be carried out in this field. The recent remarkable achievements in enzymic chemical synthesis (starch, crystalline sucrose, etc.) indicate the growing necessity for greater cooperation11 between enzymologist and chemist in the solution of many biological phenomena. 

E. J. Hehre and D. M. Hamilton, Bacterial synthesis of an amylopectin-like polysaccharide from sucrose, J. Biol. Chem., 166 (1946) 77-78. 

J. F. Robyt, Mechanism in the glucansucrase synthesis of polysaccharides and oligosaccharides from sucrose, Adv. Carbohydr. Chem. Biochem., 51 (1995) 133-168. 

It is of interest that similar enzymes, capable of exchanging glycosidic linkages, appear to be involved in the synthesis of some polysaccharides. Thus the polysaccharide dextran is formed from sucrose by enzyme preparations from Leuconostoc46 while the polysaccharide levan is produced from sucrose or raffinose by enzymes of other bacteria.49 The reactions may be written as follows ... 

MECHANISMS IN THE GLUCANSUCRASE SYNTHESIS OF POLYSACCHARIDES AND OLIGOSACCHARIDES FROM SUCROSE... 

In 1861, Louis Pasteur1 reported a polysaccharide that was produced from sucrose. In 1874, Scheubler2 determined its empirical formula and named it dextran. The formation of polysaccharide (dextran) was observed as the result of bacterial transformation of sucrose solutions into viscous solutions, gels, and/or flocculent precipitates.3 The synthesis of dextran from sucrose by a cell-free... 

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