Succinimidyl-7-amino-4-methylcoumarin-3-acetic acid,

AMCA-NHS, succinimidyl-7-amino-4-methylcoumarin-3-acetic acid, is an amine-reactive derivative of AMCA containing an NHS ester on its carboxylate group (Thermo Fisher). The result is reactivity directed toward amine-containing molecules, forming amide linkages with the AMCA fluorophore (Figure 9.23). Proteins labeled with AMCA show little-to-no effect on the isoelectric point of the molecule. [Pg.432]

Other Names 2//-l-Benzopyran-6-sulfonic acid, 7-amino-3-[2-[(2,5-dioxo-l-pyrroiidinyl)oxy]-2-oxoethyl] -4-methyl-2-oxo- 7-amino-3-((((succinimidyl)oxy)-carbonyl)methyl) -methylcoumarin-6-sulfonic acid AMCA-S Alexa 350 carboxylic acid succinimidyl ester Alexa Fluor 350 carboxy acid succinimidyl ester Alexa Fluor 350 succinimidyl ester Alexa Fluor 350NHS Sulfosuccinimidyl-7-amino-4-methylcoumarin-3-acetic acid... [Pg.15]

Alexa Fluor 350 carboxylic acid succinimidyl ester (AMCA-S) 7-Amino-4-methylcoumarin (AMC) (Coumarin 120) (Coumarin 440) 7-Anilinocoumarin-4-acetic acid (ACAA)... [Pg.437]

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