Subtilisins sequences compared

Very recently it was shown that the X-ray analysis of eglin c and its complex with subtilisin revealed a similar type of global structural changes induced by the subtilisin binding.29 Obviously SSI has no sequence or structural similarity with eglin c, which is a monomeric protein having 70 amino acid residues produced by the leech Hirudo medicinalis. It will thus be very interesting to compare the structural as well as biochemical characteristics of these two inhibitors. 

The sequence of aqualysin Il6) (AQU) is shown compared with those of proteinase Kl7) (PRO), thermitase22 (THE), subtilisin BPN 18 (BPN), and subtilisin El9) (E). Identical amino acids with those of aqualysin I are shown by hyphen (—). Open space is the position where a corresponding amino acid is absent. The numbering above the sequences refers to aqualysin I, and that below the sequences to subtilisins. Asterisks indicate the active-site residues, Asp, His, and Ser. 

---