Subject lincomycin

During the development of a sterile formulation of pirlimycin, a semisynthetic, veterinary lincosaminide antibiotic derived from lincomycin 4, three lots of material were subjected to thermal stress at 40°C for a period of 4 months, which led to the observation of a previously unreported degradant present at 0.2% [69]. 

In order to determine lincomycin hydrochloride in n - butanol extracts, the butanol solution must be diluted to four times of its original volume with ethanol, after which 0.1 - 1 ml of the resulting solution is subjected to the above mentioned steps. 

The effect of antimicrobial agents on serum folate level was studied using the standard autoclaving technique of Lactobacillus casei microbial assay. The blood for serum folate assay was obtained from adult human subjects before and 2 hours after a 24 hours test dose of the antibiotic (72). The results show that lincomycin produced a falsely low serum folate level. 

In a study in 10 healthy subjects the mean peak serum levels of a single 500-mg oral dose of lincomycin were about 3 micrograms/mL when taken 4 hours before breakfast, 2 micrograms/mL when taken 1 hour before breakfast, and less than 1 microgram/mL when taken after breakfast. The mean total amounts of lincomycin recovered from the urine were 40.4, 23.8, and 8.9 mg, respectively. ... 

About 85 mL of Kaopectate (kaolin-pectin) reduced the absorption of lincomycin 500 mg by about 90% in 8 healthy subjects. Giving the Kaopectate 2 hours before the antibacterial had little or no effect on its absorption, whereas when Kaopectate was given 2 hours after lincomycin, the absorption was reduced by about 50%. The absorption rate of clindamycin is markedly prolonged by kaolin, but the extent of its absorption remains unaffected. ... 

Further details of chemical characterization and nuclear magnetic resonance data as well as ab initio calculations on lincomycin and related structures subsequently appeared [4-10]. For early reviews on lincomycin subject see Refs. [11, 12]. 

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