Subcellular analysis cytoplasm

The third approach to subcellular analysis involves using the separation capillary to sample sub-cellular compartments within single cells or from tissue sections. Capillaries are uniquely suited to provide not only high resolution and separation efficiency but also, due to their physical dimensions, sufficient spatial resolution to sample subcellular compartments. Most commonly, the capillary is used to sample the cytoplasm from single cells. However, the capillary can also be used to sample intact organelles from tissue sections and analyze organelles released from single cells. ... 

However, it is intriguing to speculate that Mcl-l and A1 may play specialised, but overlapping roles in neutrophil survival. Mcl-l has been reported to be localised mainly to mitochondria [109,110], whereas the subcellular location of A1 is reported to be cytoplasmic [128, 129]. Indeed, sequence analysis implies that A1 lacks a defined transmembrane anchor at its carboxy terminus. If these proteins are localised to different subcellular compartments, then they could both function to protect against apoptosis, but via different pathways, perhaps by interacting with different death proteins. 

Sims, C. E., Luzzi, V., and AUbritton, N. L., Lxjcalized sampling, electrophoresis, and biosensor analysis of Xenopus laevis cytoplasm for subcellular biochemical assays. Methods Mol. Biol., 322, 413-424, 2006. 

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