Structures and Chemical Properties - Analytical Considerations

It should not be forgotten that the safety imphcations of the analyte (and/or the matrix) for the analyst is an important consideration. When indicated by available information on this aspect (e.g. a Materials Safety Data Sheet (MSDS) sheet or a published LD5Q), any proposed analytical method must take into account the safety of laboratory personnel. In extreme cases speciahzed equipment (fume hoods, glove boxes etc.) may be necessary. 

Selection of sample preparation methodologies (Section 4.3) must obviously take into account the chemical nature and properties of anal54e and matrix, but also the possibility of related compounds (metabolites, breakdown products etc.) and unrelated matrix constituents that could lead to matrix effects in the analytical step (Sections 5.3.6a and 9.6). These considerations affect the extent of necessary clean-up procedures following extraction, but of course the situation is complicated by the role of the analytical chromatography in possibly resolving 

At this first planning stage it may be advisable to consider the likely benefits of initial development of two analytical methods rather than just one, as an insurance policy against eventual failure to meet fitness for purpose criteria. Of course the price paid for this insurance involves increased initial costs and laboratory effort. 

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