Stress responses gene regulation

In the Gram-positive bacterium Bacillus subtilis (DNA with low G + C content), three Fur-like proteins have been characterized (Bsat et al., 1998). One, called Fur, regulates mainly iron uptake and siderophore biosynthesis. A second one, called PerR, regulates peroxide stress response genes and acts with manganese as corepressor. A third one, Zur, regulates genes for zinc uptake. The Zur protein found in E. coli shows only 25 % identity to the B. subtilis Zur, while the two Fur proteins have 32 % identical amino acids. 

A role for MT in mediating the zinc status of MRTFs important in regulating expression of stress response genes. 

Grandvalet, C., Coucheney, F., Beltramo, C., and Guzzo, J. (2005) CtsR is the master regulator of stress response gene expression in Oenococcus oeni. J Bacteriol 187(16), 5614-5623. 

Phillips, B. Morimoto, R.I. (1991). Transcriptional regulation of human hsp70 genes Relationship between cell growth, dilTeientiation, virus infection, and the stress response. In Results ... 

Recent studies have further examined the iron stress response of pseudomonads using an iron-regulated, ice-nucleation gene reporter (inaZ) for induction of the iron stress response (17,18,84). This particular reporter system was developed by Loper and Lindow (85) for study of microbial iron stress on plant surfaces but was later employed in soil assays. In initial. studies, cells of Pseudomonas fluorescens and P. syringae that contained the pvd-inaZ fusion were shown to express iron-responsive ice-nucleation activity in the bean rhizosphere and phyllosphere. Addition of iron to leaves or soil reduced the apparent transcription of the pvd-inaZ reporter gene, as shown by a reduction in the number of ice nuclei produced. 

---