Strains and Media

XP cells were obtained from the American Type Culture Collection. Cells with normal DNA repair were derived from foreskin material or skin biopsies 

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Strains and Media. Stock cultures of Diaporthe phaseolorum (Cke. and Ell.) var. sojae Wehm. (syn. D. sojae Leh.), Cercospora sojina Kara (syn. C. daizu), and Septoria glycines Hemmi were maintained on Potato Dextrose Agar at 25 C. 

The P-lactam antibiotics ate produced by secondary metaboHc reactions that differ from those responsible for the growth and reproduction of the microorganism. In order to enhance antibiotic synthesis, nutrients must be diverted from the primary pathways to the antibiotic biosynthetic sequences. Although most media for the production of penicillins and cephalosporins are similar, they ate individually designed for the specific requkements of the high yielding strains and the fermentation equipment used. 

Other interference-produced colors falling into this section include doubly refracting materials such as anisotropic crystals and strained isotropic media between polarizers, as in photoelastic stress analysis and in the petrological microscope. 

Streptokinase and streptodornase are isolated following growth of non-pathogenic streptococcal producer strains in media containing excess glucose. They are obtained as a crude mixture from the culture filtrate and can be prepared relatively free of each other. They are commercially available as either streptokinase injection or as a combination of streptokinase and streptodornase. 

The bacterial strains and plasmids used in this work are given in table 1. Media and culture conditions... 

Some of these are the influent oil content, the influent suspended solids content, the desired effluent quality, temperature, space, and dependability of treatment. An advantage that mixed media provides is that the size and quantity of each filter material can be varied to meet the requirement. As an example, the typical mixed-media filter bed is 30 in. deep and utilizes 60-pcrccnt anthracite coal of 1.0 mm size, 30-pcicenl sand of 2.6 S.G. and 0.5 mm size, and 10-percent garnet sand of 4.2 S.G. and 0.25 mm size. Such a filter will provide very efficient removal of suspended solids over 10 to 15 microns in size without the use of chemicals. With aluminum sulfate or other coagulants, much smaller particles can be removed. Any oil associated with these suspended solids is also removed. We believe that the removal of oil with mixed-media filters can be attributed partially to mechanical straining and partially to adsorption on the media. 

Soft biological structures exhibit finite strains and nonlinear anisotropic material response. The hydrated tissue can be viewed as a fluid-saturated porous medium or a continuum mixture of incompressible solid (s), mobile incompressible fluid (f), and three (or an arbitrary number) mobile charged species a, (3 = p,m, b). A mixed Electro-Mechano-Chemical-Porous-Media-Transport or EMCPMT theory (previously denoted as the LMPHETS theory) is presented with (a) primary fields (continuous at material interfaces) displacements, Ui and generalized potentials, ifi ( , r/ = /, e, to, b) and (b) secondary fields (discontinuous) pore fluid pressure, pf electrical potential, /7e and species concentration (molarity), ca = dna/dVf or apparent concentration, ca = nca and c = Jnca = dna/dVo. The porosity, n = 1 — J-1(l — no) and no = no(Xi) = dVj/dVo for a fluid-saturated solid. Fixed charge density (FCD) in the solid is defined as cF = dnF/dV , cF = ncF, and cF = cF (Xf = JncF = dnF/d o. 

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