Stationary phases effects

The degree to which the ion-exchange stationary phase can interact with the solute molecules is affected by pH. At pH values above the p a of ion-exchange groups, anion-exchangers are neutraUsed by base  

A wide variety of ion-exchange packings are commercially available from such companies as Pharmacia, SynChropak, Toyo Soda, Bio-Rad, Whatman and Varian. They can generally be divided into 4 classes  

---

Peyrin, E. et al., Dansyl amino acid enantiomer separation on a teicoplanin chiral stationary phase effect of eluent pH, J. Chromatogr. A, 923, 37, 2001. 

Orendorff, C.J. and Pemberton, J.E., Raman spectroscopic study of the conformational order of octadecylsUane stationary phases effects of electrolyte and pH, Anal. Bioanal. Chem., 382, 691, 2005. 

These equations show the order of magnitude of the optimum point for the case in which stationary phase effects are negligible. We observe that the minimum H differs only by a numerical constant from the particle diameter... 

Figure 12.4. Plate height versus flow velocity plot for negligible stationary phase nonequilibrium effects (bottom curve) and for dominant stationary phase effects (top).

N. Tanaka, K. Kimata, K. Hosoya, H. Miyanishi, and T. Araki, Stationary phase effects in reversed-phase liquid chromatography /. Chromatogr. A 656 (1993), 265-287. 

Stationary phase effects in normal-phase liquid chromatography In normal-phase liquid chromatography the stationary phase is typically a... 

Stationary phase effects in reversed-phase liquid chromatography... 

Colin, H., Krstulovic, A., Guiochon, C. and Yun, Z. (1983). Stationary Phase Effects in Reversed-Phase Liquid Chromatography. J. Chromatogr., 255, 295. 

Ion exchange. This postulates that the lipophilic tail of the counter-ions locates onto the bonded stationary phase, effectively causing the column to behave as an ion exchanger. 

On the other hand solvophobic theory attributes stationary phase effects to solute-ligand interactions in the gas phase and is unable to account for silanophilic interactions... 

In addition to solvophobic and silanophilic interactions other stationary phase effects, including steric recognition and pi-pi interactions between solutes and stationary phase, have been demonstrated. Thus, the specific nature of the stationary phase and solutes in-... 

The solvent reservoir contains the mobile phase, which is typically made up of mixtures containing water and an organic modifier such as acetonitrile or methanol and/or buffer solutions. However, complex separations may require more complex mobile phase compositions. The mobile phase assists in the transportation of compounds in a mixture through the stationary phase, effecting separation into the individual components. This will be further discussed in Chapters 3 and 4. 

Xiao, Y.Z., Jones, T.T., Laurent, A.H., et al. (2007) Protein instability during HIC Hydrogen exchange labeling analysis and a framework for describing mobile and stationary phase effects. Biotechnol Bioeng, 96 (1), 80-93. 

S. Yang and M. Khaledi, Stationary Phase Effects on Retention of Phenols in MLC Perfluorooctane versus C18, Anal. Chim. Acta, 294 135 (1994). 

Using PDA Spectroscopy for Evaluating Eluent and Stationary Phase Effects on Protein Structure... 

Booth TD, Lough WJ, Saeed M, Noctor TAG, Wainer IW (1997) Enantioselective separation of enantiomeric amides on three amylose-based chiral stationary phases effects of backbone and carbamate side chain chiralities. Chirality 9 173-177... 

Direct separation by using chiral stationary phases, effected by the formation of diastereomeric association complexes... 

---