Starch enzymatic analysis

Probably the most important carbohydrate polymer in food, certainly from a nutritional standpoint, is starch, and Chapter E2 presents three units that can be used for starch isolation and characterization. UNITE2.1 condenses many different starch isolation methods into one scheme for starch isolation from virtually any plant source. In this protocol, the author notes the care that must be taken to prevent starch degradation during isolation. unite2.2 presents a simple enzymatic analysis method that can be used for estimation of starch in food. Finally, unit E2.3 describes a colorimetric method to determine starch amylose content. Again, different combinations of the units in this chapter can be used to characterize starch from any source. 

NIRA, enzymatic analysis of total starch (AACC 76-11 and 13)... 

Damaged Starch in Flours Analysis by Enzymatic Vs Imaging Methods... 

Gel filtration of the hemicellulose was done on a G-50 Sephadex column with water as eluent by the National Food Research Institute of the CSIR. Neutralized samples were applied to the column and fractions (6 mL) collected, of which 1-mL aliquots were assayed by the phenol-sulfuric acid test ( ). Approximate calibration (D.P. versus elution volume on the G-50 column) was done by total carbohydrate and reducing sugar end group analysis (0) using enzymatically debranched starch as reference material. 

In spite of all these difficulties, microdialysis has been successftilly coupled to several analytical techniques for the bioprocess monitoring. HPLC is commonly used and such systems have been apphed in the analysis of oligosaccharides [44,180], ethanol [181], enzymatic digestion of lactose in milk [182], studies on drug dissolution [183], saccharides in wastewater [184], starch hydrolysis products [185]. 

In order to describe the kinetics of enzymatic starch depolymerization, information on reaction rate, reaction extent, and product distribution profiles are required. Traditional end-group analysis can be used to a limited extent in the first two areas, but will not provide information about the last important subject. Hence, SEC profiles can provide sufficient insight into the mechanism of starch degradation. 

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