SsDNAs

Collectively, these thermal denaturation studies demonstrated that aPNAs bind to complementary ssDNA targets with high affinity and in a sequence-specific manner consistent with our proposed base-pairing model. Additional electrostatic and hydrophobic binding interactions can be incorporated into the aPNA design without affecting the primary Watson-Crick binding mode. 

These gel retardation studies provide unambiguous support for the formation of a 1 1 complex between the b2 aPNAs and ssDNA. The dissociation constant (fQ)) for these complexes was determined to be in the hundred nanomolar range. In the case of homothymine b2 aPNA dimers, an additional 2 1 complex is also evident. 

Unwinding (denaturation) of dsDNA to provide an ssDNA template. 

Molecular recognition 113 0 t Q Protein, ssDNA, dsDNA, tRNA, rRNA, mRNA, nRNA, bilayers, ligands, cofactors, metals, autoregulatory... 

Fluorescence studies and the binding interaction of Quartz/APES/RB with single- and double-stranded oligonucleotides (ssDNA and dsDNA) in Tris-HCl buffer solution of pH 7.4 were carried out. Quartz/APES/RB exhibited emission at 576 nm, whereas Quartz/APES without BB where nonfluorescent, suggesting that RB successfully assembled on the surface of quartz wafers. By comparison with Aem of 5 x 10 5 M RB solution, which was 588 nm, a hypsochromic shift was found. Considering the fluorescence microscopical image of Quartz/APES/RB, it... 

Overall, Quartz/APES/RB allowed for extremely high sensitive fluorescence recognition for ssDNA and dsDNA with the detection limit of 2.4 ngL 1 and 0.85 ngL-1, respectively. 

Golden M.C., Collins B.D., Willis M.C., Koch T.H., Diagnostic potential of PhotoSELEX-evolved ssDNA aptamers, J. Biotechnol. 2000 81 167-178. 

Proteins can be immobilized on the cell surface with the use of a short, single stranded DNA (ssDNA) attached to the end of a PEG chain (ssDNA-PEG-lipid) [114—116, 119, 125]. First, an ssDNA-PEG-lipid is prepared by conjugating... 

Fig. 8 Immobilization of urokinase on the surfaces of islet cells, (a) Surface modification (/) chemical structure of ssDNA-PEG-lipid, and (2) ssDNA-PEG-lipid anchoring to the cell membrane. (b) Introduction of a complementary ssDNA onto urokinase, which was first modified with a madeimide group by a cross-linker, EMCS. (c) Urokinase-immobilization through DNA...

Fig. 9 Surface modification of cells with ssDNA-PEG-lipid. (a) Real-time monitoring of PEG-lipid incorporation into a supported lipid membrane by SPR. (r) A suspension of small unilamellar vesicles (SUV) of egg yolk lecithin (70 pg/mL) was applied to a CH3-SAM surface. A PEG-lipid solution (100 pg/mL) was then applied, (ii) Three types of PEG-lipids were compared PEG-DMPE (C14), PEG-DPPE (C16), and PEG-DSPE (C18) with acyl chains of 14, 16, and 18 carbons, respectively, (b) Confocal laser scanning microscopic image of an CCRF-CEM cell displays immobilized FITC-oligo(dA)2o hybridized to membrane-incorporated oligo(dT)20-PEG-lipid. (c) SPR sensorigrams of interaction between oligo(dA)2o-urokinase and the oligo (dT)2o-PEG-lipid incorporated into the cell surface, (i) BSA solution was applied to block nonspecific sites on the oligo(dT)20-incorporated substrate, (ii) Oligo(dA)20-urokinase (solid line) or oligo(dT)20-urokinase (dotted line) was applied...

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