SRM chromatograms

Figure 1 SRM chromatograms of (a) fenamiphos and metabolites and (b) imidacloprid and metabolites. IS refers to the stable labeled isotopes. The values below the names refer to the mass transitions, i.e., M -b 1 -> product ion for the metabolite...

Fig.4 Reconstructed SRM chromatograms obtained from the LC-ESI-MS/MS analysis of a 100 ng mL-1 standard mixture of estrogens (in the NI mode) and progestogens (in the PI mode). Column Purospher STAR RP-18e (125x2 mm, 5 pm, Merck). Mobile phase gradient acetonitrile/water. Flow rate 0.2 mL min-1...

FIGURE 18.3 Time-scheduled SRM chromatograms of carbamazepine and its metabolites in an effluent from the STP of Perborough (a) 10,11-dihydroxycarbamazepine (CBZ-DiH), internal standard (b) carbamazepine (CBZ) (c) 3-hydroxycarbamazepine (d)10,ll-dihydro-10,ll-epoxycarbamazepine (e) 2-hydroxycarbamazepine (f) 10,ll-dihydro-10-hydrocarbamazepine and (g) 10,ll-dihydro-10,ll-dihydroxy-carbamazepine. (Reprinted from Petrovic, M. et ah, J. Chromatogr. A, 1067, 1, 2005. Copyright 2005. With permission from Elsevier.)... 

Figure 1.31. LC-MS/MS (SRM) chromatograms for muraglitazar (517—>186) in human plasma obtained using (a) HPLC and (b) HILIC. (Reprinted with permission from Xue et al., 2006)...

Figure 6.36 Representative LC/MS/MS SRM chromatograms obtained from the analysis of an extract of a 50pg/mL calibration standard. (A) Chromatogram for the m/z 410 precursor to m/z 120 product ion of clozapine. (.B) Chromatogram of the m/z 416 precursor to m/z 126 product ion of 13C6-clozapine [internal standard], (Reprinted with permission from Dear et al., 1998. Copyright 1998 Elsevier.)...

Figure 15.4. LC/ESI-MS/MS SRM chromatograms of authentic polycavemosides and the semi-purified toxic fraction from G. edu/is. (A) A volume of 10 pi of the standard mixture containing 120 pmol of PA, 60 pmol of PA2, and 180 pmol of PA3 and 300 pmol of PB2 (rmlz 821-483, 833-513, 847-513, 861-513). (B) An aliquot of the semi-purified toxic fraction obtained from the causative G. edu//s(m/z 847-513, 847-673 for PA). The ion intensity of the base peak (100% relative intensity) was indicated in each mass chromatogram. The chromatographic condition was the same as that in Figure 1 5.2.

FIGURE 1.13 SRM chromatograms obtained from a four compound mixture analysis based on a single injection assay. In this case, the mass spectrometer was switching between the four ionization modes (+/- ESI and APCI) rapidly. Approximately 15 data points were obtained for each peak. (Reprinted from Yu, K. et al., Rapid Commun. Mass Spectrom., 21, 893, 2007. With permission.)... 

FIGURE 4.11 SIM and SRM chromatograms for the same sample. In the SIM mode (A), several interference peaks are observed while in the SRM mode (B), only the analyte is apparent. (Adapted from Fung, E.N. et al., Rapid Commun. Mass Spectrom., 17, 2147, 2003. With permission.)... 

FIGURE 28.9 LC-ESI/MS/MS SRM chromatograms of authentic polycavernosides (a) and the semipurified toxic fraction from G. edulis (b). The chromatographic condition was the same as that in Figure 28.7. 

FIGURE 6.4 The SRM chromatogram of the adult/infant milk formula (standard reference material 1849a). 

FIGURE 9.11 UHPLC-ESI(+)-TOF EICs at 302.0252 of (a) an olive oil sample where phosmet-oxon was detected (PHOS-Ml) and (b) phosmet-oxon standard at 100 ng/mL. HPLC-ESI(+)-QqQ SRM chromatograms of (c) the same olive oil sample than in (a), and (d) phosmet-oxon standard at 1 ng/mL in olive oil matrix. (From Hernindez, F. et al. 2009. / Sep. Sci. 32 2245-2261. With permission.)... 

However, an enhanced resolution quadrupole (Schoen 2001 Yang 2002) can in principle provide increased selectivity in quantitative analyses using SIM or MRM. An example in which this advantage is clearly evident is shown in Figure 6.12, where the SRM chromatogram obtained using a RP yielding a peak width (FWHM) of... 

Figure 6.12 Comparison of the selectivities exhibited in SRM chromatograms m/z 470.22 274) obtained at FWHM settings of 0.70 (top) and 0.20 (bottom) Th for Q, (precursor ion selection) in LC-MS/MS analyses using the same enhanced-resolution triple quadrupole instrument. In each case the sample was human urine spiked at 30pg.mL of nefazodone sample in human urine, with 60 fg injected on column. Reproduced from Jemal, Rapid Common. Mass Spectrom. 17, 24 (2003), with permission of John Wiley Sons, Ltd.

Figure 11.10 HILIC-MS/MS analyses (MRM mode) of an extract of (left side) a plankton sample (Alexandrium tamarense) and (right side) of tissue from contaminated mussels (Mytilus edulis), each containing several PSP toxins. HILIC conditions were the same as those used with analytical standards to obtain Figure 11.9. Some SRM chromatograms are plotted with expanded scales as indicated. Reproduced from Dell Aversano, /. Chromatogr. A, 1081, 190 (2005), cop)rtght (2005) with permission from Elsevier.

Figure 8.114 Suppressed conductivity and SRM chromatograms of endothaii spiked into different water sampies. Separator coiumn lonPac AS16 with guard coiumn dimensions 250 mm X 2 mm i.d. coiumn temperature ...

Figure 8.124 SRM chromatogram of a urine sample spiked with native and labeled EDEA, MDEA, and TEA. Separator column BDS Hypersil Cyano, 3 pm column dimensions ...

LC-MS detection of 100 pg dextrometorphan (DEX) spiked into 1 ml of human plasma. The corresponding signal of the [M+H]" ion in toe SIM trace at m/z 272 is barely detectable, whereas toe SRM chromatogram obtained from the reaction [M+H]" -> [M-CgHisN]" shows a clean background and a signal-to-noise enhancement of more than 50-fold (Fig. 14.19) [27]. 

Figure 4.54 Calibration curve (0.1-100ppb) and SRM chromatogram for propazine (1 ppb).

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