Spot test reagents

This spot-test reagent is now supplied water-wet since it has been found to be detonable and legally classifiable as an explosive. The editor has not heard of any problems in use. 

The flavone morin (700) (Natural Yellow C.I. 75660) dyes wool yellow, the actual shade being determined by the mordant which is used. It has also been mentioned as a spot test reagent for salts of aluminum, beryllium, zinc and other metals (B-54MI22401). 

Solvent of arystallization, 42 Solvents distilling off, 21 for recrystallization, 35 Spiral gas washing bottle, 62 Spot test reagents, 396 SS acid (Chicago add), see 1-Amino-8-naphthol 2,4-disulfonic add... 

Lev Aleksandrovich Chugaev Dimethylglyoximate test for nickel (first organic spot test reagent for a metal ion)... 

Use Mordant dye, spot-test reagent for metal salts, luminescence indicator. 

USE Intermediate for dyestuffs and drugs spot test reagent for iron. 

USE Spot -test reagent for detection of copper, mercury, zinc F. J. WeJcher, Organic Analytical Reagents, vol, IV... 

Feigl, F. and V. Anger, Replacement of benzidine by copper ethy-acetoacetate and tetra base as spot-test reagent for hydrogen cyanide and cyanogen, Analyst, 91, 282-284 (1966). 

Stationary phase Binder free Zirconium (IV) antimonate ion exchanger in H form, mixture of zirconium antimonate and silica gel (1 1) and silica gel. Mobile phase HNO, (10 -1.0 M), DMSO-O.I M HN03(10 0, 8 2, 6 4, 4 6, and 2 8), dioxane-0.1 M HNO, 8.2, 6 4, 4 6, and 2 8). Conditions Ascending technique, layer thickness 0.1 mm. Detection Standard spot test reagents. Remarks Quantitative separation of Ru (5-50 pg) from synthetic mixtures of metal ions. Ru after detection with 2% thiourea solution in 2 M HCI was eluted from working plate with 0.1 M HCl, filtered and determined spectrophotometrically applying the p-nitrosodimethylaniline method. The standard deviation was 8-25 ppt. 

Stationary phase Silica gel G. Mobile phase M, M2, Afj, and M4 are 0.1 M solutions of trichloroacetic, dichloroacetic, monochloroacetic and acetic acids respectively. Detection Standard spot test reagents. Remarks The effects of concentration, pH and chlorosubstitueni groups of the mobile phase on the Rf values of metal ions were examined. 

Ascending technique, activation at 100 5°C for 2 h. run 11 cm. The activated silica gel layers were impregnated with 1 1 mixture of DMSO and sulphur free toluene, heated in an oven till the dampness is disappeared and cooled in an air tight glass cabinet before use. Detection Conventional spot test reagents. Remarks The R, values of transition metals on impregnated layers developed with DMSO-THF (1 10) were proportional to their atomic numbers. 

Stationary phase Silica gel G (E. Merck). Mobile phase 0.01 M Aqueous solution of sodium thioglycolate (pH = 2.0). Detection Standard spot test reagents. Conditions Ascending technique, silica gel slurry applied on glass plates (4 x 10 cm) by dipping method, plates dried overnight at room temperature before use, all experiments at room temperature, development time Di = 5 min D2 = 20 min. Remarks The dependence of Rf values on development time, pH value and the concentration of mobile phase was examined. The optimum conditions for ternary separations, and quantitative determination by AAS were established. Application of the developed method for determination of Zni in human viscera. 

DMF -t- 2 M HCl My — DMF -1- 6 M HNO3. Conditions Ascending technique, run 14 cm, layer thickness 0.1 mm on 20 x 20 cm glass plates, loading volume 0.04 ml. Detection Conventional spot test reagents. Remarks Quantitative separation of UO2 from a synthetic mixture of other metal ions. 

HCI-n-butanol-acetone (3 1 6) W2S = HCl-n-pentanol-acetone (3 1 6) W27 = HCl-n-hexanol-acetone(3 1 6) M28-M.M = HCl-DMSO-piopanol-2 in 1 1 1, 2 1 1, 1 2 1, and 1 1 2 ratio respectively. Detection Conventional spot test reagents used for detection of Cu, Ni, Coi, 2j, Mn, and Fe . Conditions Ascending technique, run 10 cm. layer thickness 0.25 mm. plate activation at 110 2°C for I h. loading volume 10 Remarks Qualitative separation of transition metal chlorosulphates and quantitative spectrophotometric determination of cobalt chlorosulphate were discussed. Source A. Mohammad, K. T. Nasim, J. Ahmad, and M. Najar P. A.. Analusis. 23 243 (1995). 

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