Spot reduction, method

The final consideration in suction piping design is the method used to reduce the pipe diameter (i.e. 1.5 or greater than the pump s inlet flange) so that it can be attached to the pump. Care must be taken to ensure that the reduction method does not cause a void where air can be trapped or a low spot that will permit buildup of solid contaminates. 

Spot Reduction Spot reduction is a basic form of print retouching. Spot reduction is useful for the removal of small pinhole spots or other small areas that need to be lightened. The spot method allows the photographer to add pleasing catch lights to eyes and to remove an occasional unwanted element from the image. 

Instructions for Spot Reduction I was originally taught spot reduction by photographer Boyd Wetlauffer of Canada in 1974. Boyd always used a wet print. I prefer to use a dry print as I have found spotting a wet print hard to control. Try both methods, and see which works best for you. 

If the drill string becomes differentially stuck, mechanical methods or spotting fluids can be appHed, or the hydrostatic pressure can be reduced (147). In general, penetration of water- or oil-based spotting fluids into the interface between the filter cake and the pipe accompanied by dehydration and cracking results in reduction of differential pressure across the drill string (147,148). Spotting fluids are usually positioned in the open hole to completely cover the problem area. 

The XPS spectra were recorded on a Surface Science Laboratories small spot system using a monochromatized A1K X-ray radiation source. The take-off angle used for these measurements was 35°. Full details of the methods used in interpreting the XPS data have been described elsewhere [14], Data reduction was done using Surface Science Laboratories software version 8.0. This software utilizes a least squares curve fitting approach with only chi square statistics for goodness of the calculated fit to the experimental data. 

Matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF)-mass spectrometry (MS) is now routinely used in many laboratories for the rapid and sensitive identification of proteins by peptide mass fingerprinting (PMF). We describe a simple protocol that can be performed in a standard biochemistry laboratory, whereby proteins separated by one- or two-dimensional gel electrophoresis can be identified at femtomole levels. The procedure involves excision of the spot or band from the gel, washing and de-stain-ing, reduction and alkylation, in-gel trypsin digestion, MALDI-TOF MS of the tryptic peptides, and database searching of the PMF data. Up to 96 protein samples can easily be manually processed at one time by this method. 

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