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Splenocyte proliferation assays

Fig. 1. A -THC enhances CB2R" splenocyte proliferation, but not CB2R spleno-cyte proliferation. Splenocyte proliferation was determined by BrdU incorporation assay as described in the text. Cells were plated at 2 x 10 cells/0.1 mL/well in complete media. Cells were treated with 2.5 pg/mL ConA and the indicated A -THC concentration for 72 h. Data are expressed as the mean of qnadruplicate samples standard deviation. Vehicle is absolute ethanol. Fig. 1. A -THC enhances CB2R" splenocyte proliferation, but not CB2R spleno-cyte proliferation. Splenocyte proliferation was determined by BrdU incorporation assay as described in the text. Cells were plated at 2 x 10 cells/0.1 mL/well in complete media. Cells were treated with 2.5 pg/mL ConA and the indicated A -THC concentration for 72 h. Data are expressed as the mean of qnadruplicate samples standard deviation. Vehicle is absolute ethanol.
Four polysaccharides (FCp-1, FCp-2, FCp-3 and FCp-4) were isolated from citrus fruits (Citrus medica L. var. sarcodactylis) after hot-water extraction and ethanol precipitation. Among all, only the FCp-3, a polysaccharide with a molecular weight of 177.1 KDa, showed an immunological activity evaluated by splenocyte and thymocyte proliferation assay. FCp-3 displayed a significant splenocyte proliferation at a dose of > 25 microg/mL, and showed a moderate effect on thymocyte proliferation at the same dose [70]. [Pg.12]

See other pages where Splenocyte proliferation assays is mentioned: [Pg.395]    [Pg.179]    [Pg.23]    [Pg.31]    [Pg.174]    [Pg.453]    [Pg.386]    [Pg.391]    [Pg.409]    [Pg.268]    [Pg.419]   
See also in sourсe #XX -- [ Pg.23 , Pg.31 ]



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