Spin-labelled ESR

Lateral diffusion of phospholipids in model membranes at ambient pressure has been studied over the years by a variety of techniques including fluorescence recovery after photobleaching (FRAP), spin-label ESR, pulse field gradient NMR (PFG-NMR), quasielastic neutron scattering (QENS), excimer fluorescence and others.In general, the values reported for the lateral diffusion coefficient (D) range from 10 to 10 cm /s in the... [Pg.190]

Mchaourab, H.S. and Perozo, E. (2000) Determination of proteins folds and conformational dynamics using spin-labeling ESR spectroscopy, in Berliner, L, Eaton, S., and Eaton, G. (eds.),, Magnetic Resonance in Biology, V. 18, Kluwer Academic Publishers. Dordrecht, pp. 185-248. [Pg.211]

Watanabe Y, Inanami O, Horiuchi M, Hiraoka W, Shimoyama Y, Inagaki F, Kuwabara M (2006) Identification of pH-sensitive regions in the mouse prion by the cysteine-scanning spin-labeling ESR technique. Biochem Biophys Res Commun 350 549... [Pg.194]

The character of spin label ESR spectra changes dramatically as a function of concentration. Even though accurate modeling of the concentration effect depends upon sorting out a number of possible-mechanisms,... [Pg.332]

Murakami K, Kara H, Masuda Y, Ohigashi H, Me K (2007) Distance measurement between TyrlO and Met35 in amyloid beta by site-directed spin-labeling ESR spectroscopy implications for the stronger neurotoxicity of A beta 42 than A beta 40. Chembiochem 8 2308-2314... [Pg.118]

Ramakrishnan M, Jensen PH, Marsh D (2006) Association of alpha-synuclein and mutants with lipid membranes spin-label ESR and polarized IR. Biochemistry 45(10) 3386-3395. doi 10.1021/bi052344d... [Pg.120]

Bordignon E, Steinhoff HJ (2007) Membrane protein structure and dynamics studied by site-directed spin-labeling ESR. In Hemminga MA, Berliner LJ (eds) ESR spectroscopy in membrane biophysics, vol 27. Biological magnetic resonance. Springer, New York,... [Pg.154]

The heterogeneous structure of the swollen B. mori silk fibroin membrane was clarified from the complicated ESR spectra of the spin-labeled silk fibroin membranes. Spin-label ESR methods are useful for dynamic analysis of silk fibroin because of the inherent high sensitivity of ESR observation and the wide detectable range of the motion of the spin-labeled site, from 10 to lO " s. The hydroxy group of the Tyr side-chain is active and thus it can be labeled with nitroxide radical compounds as shown in Fig. 31. ... [Pg.140]

An advantage of spin-label ESR studies is that they can be carried out at temperatures relevant to physiology they are therefore sensitive to molecular motion. Spin labeling permits the study of the dynamics, folding, and structure of proteins by ESR. [Pg.223]

J. Pilar, Local Dynamics of Polymers in Solution by Spin-Label ESR , in... [Pg.37]

LOCAL DYNAMICS OF POLYMERS IN SOLUTION BY SPIN-LABEL ESR... [Pg.133]

Membrane Protein Structure and Dynamics Studied by Site-Directed Spin-Labeling ESR... [Pg.648]

Fretten, P., Morris, S.J., Watts, A., and Marsh, D., 1980, Lipid-lipid and lipid-protein interactions in chromaffin granule membranes. A spin label ESR study, Biochim. Biophys. Acta, 598 247. [Pg.177]

Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...