Sphingomyelin mass spectrometry

Karlsson AA, Michelsen P, Odham G. 1998. Molecular species of sphingomyelin determination by high-performance liquid chromatography/mass spectrometry with electrospray and high-performance liquid chromatography/tandem mass spectrometry with atmospheric pressure chemical ionization. J Mass Spectrom 33 1192. 

Cool the tubes and add 75 (jlI of 1M KOH in methanol, sonicate and incubate for 2 hr at 37°C. This step removes most of the interfering glycerolipids, in particular phosphatidylcholines, that can mask sphingomyelins in a simple Mass spectrometry scan. 

Zheng L, McQuaw C, Ewing A, Winograd N (2007) Sphingomyelin/phosphatidylcholine and cholesterol interactions studied by imaging mass spectrometry. J Am Chem Soc 129(51) 15730-15731. doi 10.1021/ja0741675... 

Fast atom bombardment-mass spectrometry has been used to a limited extent in characterizing sphingomyelin. In a thioglycerol matrix, the following major ions were detected MH+, 731 M-28, 703 m/z 239, CH3(CH2)12CH=CH—CH+ m/z 184, Ophosphocholine (Dr. S. Weintraub, personal communication). 

Kerwin, J.L., Tuininga, A.R., Ericsson, L.H. 1994. Identification of molecular species of glycerophospholipids and sphingomyelin using electrospray mass spectrometry. J. Lipid Res., 35, 1102-1114. 

Byrdwell WC, Perry RH. Liquid chromatography with dual parallel mass spectrometry and (31)P nuclear magnetic resonance spectroscopy for analysis of sphingomyelin and dihydrosphingomyelin. 1. Bovine brain and chicken egg yolk. J. Chromatogr. A 2006 1133 149-171. 

Liebisch G, Lieser B, Rathenberg J, et al. (2004) High-throughput quantification of phosphatidylcholine and sphingomyelin by electrospray ionization tandem mass spectrometry coupled with isotope correction algorithm. Biochim Biophys Acta 1686 108-117... 

Karlsson, A.A., Amoldsson, K.C., Westerdahl, G., and Odham, G., Common molecular species of glucosyl ceramides, lactosyl ceramides and sphingomyelins in bovine milk determined by high-performance liquid chromatography-mass spectrometry, Milchwissenschaft, 52,554—559,1997. 

Valeur, A., Olsson, N. U., Kaufmann, P. et al. (1994) Quantification and comparison of some natural sphingomyelins by on-line HPLC/discharge assisted thermospray mass spectrometry. Biol Mass Spectrom., 23, 313-19. 

Figure 9.12 Direct electrospray ionization-mass spectrometry analysis of human erythrocyte plasma membrane phospholipids (A) A positive-ion electrospray ionization (ESI) mass spectrum of erythrocyte plasma membrane phospholipid extract showing 14 molecular species of glycerophospholipids and 4 molecular species of sphingomyelin (B) A negative-ion ESI mass spectrum of the same extract of plasma membrane phospholipids showing more than 25 molecular species of ethanolamine glycerophospholipids and 8 molecular species of serine and inositol glycerophospholipids. Reprinted with permission from Han, X. and Gross, R. W., Electrospray ionization mass spectroscopic analysis of human erythrocyte plasma membrane phospholipids, Proc. Natl Acad. Scl USA, 91(22), 10635-9. Copyright (1994) National Academy of Sciences, USA.

We describe the utility of intermediate-pressure MALDI and tandem mass spectrometry (MS/MS and MS ) for the characterization and imaging of phospholipids in brain tissue sections. The use of both MS/MS spectra and MS/MS images allows for identification of isobaric compounds. The structural characterization of phosphatidylcholines, phosphatidylserines, phosphatidylethanolamines, and sphingomyelins directly fi om tissue sections is described. 

Hsu, F.-F., Turk, J. (2000) Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization. J Am Sot Mass Spectrom, 11, 437—449. 

Hsu, F.F. and Turk, J. (2000) Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization. J. Am. Soc. Mass Spectrom. 11, 437 149. Hsu, F.F. and Turk, J. (2001) Structural determination of glycosphingolipids as lithiated adducts by electrospray ionization mass spectrometry using low-energy collisional-activated dissociation on a triple stage quadrupole instrument. J. Am. Soc. Mass Spectrom. 12, 61-79. 

Sullards, M.C. (2000) Analysis of sphingomyelin, glucosylceramide, ceramide, sphingo-sine, and sphingosine 1-phosphate by tandem mass spectrometry. Methods Enzymol. 312, 32-45. 

Zhou, L., Zhao, M., Ennahar, S., Bindler, F. and Marchioni, E. (2012) Liquid chromatography-tandem mass spectrometry for the determination of sphingomyelin species from calf brain, ox liver, egg yolk, and krUl oil. J. Agric. Food Chem. 60,293-298. 

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