Spectroscopy resonance cell

D. L. Morse, N. Raghunand, P. Sadarangani, S. Murthi, C. Job, S. Day, C. Howison and R. J. Gillies, Response of choline metabolites to docetaxel therapy is quantified in vivo by localized P MRS of human breast cancer xenografts and in vitro by high-resolution P NMR spectroscopy of cell extracts. Magn. Reson. Med., 2007, 58, 270-280. 

A further example are Laser Raman spectroscopy resonance studies of the enzyme aldolase catalyzing a key reaction in the muscle cells. Zerbi et al.71) investigated resonance Raman spectra of labelled aldolase with argon ion and krypton ion lasers. 

Figure 16.10. The cell used in ion-cyclotron resonance spectroscopy. The cell is located in a vacuum chamber, placed between the poles of an electromagnet with the E and H fields as shown by the axes.

Especially useful for the investigation of ICPs and the polaron-bipolaron state was a combination of UV/vis spectroscopy and electron-spin-resonance (ESR) spectroscopy. The cell developed by Petr, Dunsch, and Neudeck is an example for the experimental verification and is shown in Figure 11.27. 

In addition to the Rabi-type experiments, a second important method of modem microwave spectroscopy was introduced in the 1930s. Gases confined in closed resonance cells were investigated by measuring the absorption of a propagating microwave field as a function of its frequency (see Figure 4). Since no pumping mechanism or other state selection was... 

The technique to shift molecular transitions into coincidence with fixed frequencies of a laser is called laser magnetic resonance (LMR). This type of spectroscopy, which is used for studying unstable molecules, was combined with the double resonance technique by Lowe and McKellar. A radiofrequency double resonance cell was placed in the 6-cm gap of the electromagnet of an intracavity laser magnetic resonance apparatus. The Zeeman magnetic field was oriented parallel to the laser light polarization (AM = 0 infrared transitions) and perpendicular to the electric field vector... 

Mclver R T 1970 A trapped ion analyzer cell for ion cyclotron resonance spectroscopy Rev. Sc/. Instrum. 41 555-8... 

With recent developments in analytical instrumentation these criteria are being increasingly fulfilled by physicochemical spectroscopic approaches, often referred to as whole-organism fingerprinting methods.910 Such methods involve the concurrent measurement of large numbers of spectral characters that together reflect the overall cell composition. Examples of the most popular methods used in the 20th century include pyrolysis mass spectrometry (PyMS),11,12 Fourier transform-infrared spectrometry (FT-IR), and UV resonance Raman spectroscopy.16,17 The PyMS technique... 

Various optical detection methods have been used to measure pH in vivo. Fluorescence ratio imaging microscopy using an inverted microscope was used to determine intracellular pH in tumor cells [5], NMR spectroscopy was used to continuously monitor temperature-induced pH changes in fish to study the role of intracellular pH in the maintenance of protein function [27], Additionally, NMR spectroscopy was used to map in-vivo extracellular pH in rat brain gliomas [3], Electron spin resonance (ESR), which is operated at a lower resonance, has been adapted for in-vivo pH measurements because it provides a sufficient RF penetration for deep body organs [28], The non-destructive determination of tissue pH using near-infrared diffuse reflectance spectroscopy (NIRS) has been employed for pH measurements in the muscle during... 

Reckel S, Hansel R, Lohr F, Dotsch V (2007) In-cell NMR spectroscopy. Prog Nucl Magn Reson Spectrosc 51 91-101... 

Calliste CA, Trouillas P, Allais DP, Simon A and Duroux JL. 2001. Free radical scavenging activities measured by electron spin resonance spectroscopy and B16 cell antiproliferative behaviors of seven plants. J Agric Food Chem 49(7) 3321-3327. 

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